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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1919-1927
DOI: https://doi.org/10.20546/ijcmas.2019.809.222


Investigation on Genetic Analysis of Safflower (Carthamus tinctorius L.) Genotypes using Rapd Molecular Markers
S. L. Dhare*, P. B. Wadikar, M. R. Magar and S. J. Sonawane
Department of Agricultural Botany, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani-431 402 (MS), India
*Corresponding author
Abstract:

Knowledge of genetic diversity and relationship among breeding material at molecular level has significant impact on safflower improvement. Genetic diversity of eight parental genotypes of safflower (Carthamus tinctorius L.) was investigated at DNA level with Random Amplified Polymorphic DNA (RAPD) procedure. Forty RAPD primers generated 943 alleles, out of which 679 alleles were found polymorphic, resulting 72.00 per cent polymorphism. The similarity coefficient ranged from 0.42 to 0.76 with an average of 0.59, thus suggesting considerable genetic variation among the safflower genotypes studied. Further, the dendrogram generated by UPGMA cluster analysis based on Jaccard’s similarity coefficient grouped eight parental genotypes into two clusters viz., cluster A and cluster B. Maximum number (7 out of 8) cultivars fell in cluster A. Cluster A was further sub divided in sub cluster A1 and A2. Least similarity of (0.42) demonstrated by GMU-2720 and EC-757665 followed by PBN-96 and EC-757665 as well as by NARI-6 and EC-757665. Genotypes, GMU-2720 and PBNS-12, GMU-1339 and PBNS-12 were found to be (0.76) per cent similar based on molecular analysis, but could not consider as duplicate. It may be due to narrowness of the genetic base of widely grown safflower and the results may be different if large number of RAPD primers may use in the study. Our result suggests that, RAPD molecular markers can detect high polymorphism and efficient for assuring genetic diversity and relatedness and also aid in selection of diverse outstanding lines to be used in future breeding programs of safflower.


Keywords: Safflower genotypes, Genetic diversity, RAPD.
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How to cite this article:

Dhare, S. L., P. B. Wadikar, M. R. Magar and Sonawane, S. J. 2019. Investigation on Genetic Analysis of Safflower (carthamus tinctorius l.) Genotypes using Rapd Molecular Markers..Int.J.Curr.Microbiol.App.Sci. 8(9): 1919-1927. doi: https://doi.org/10.20546/ijcmas.2019.809.222