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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1160-1175
DOI: https://doi.org/10.20546/ijcmas.2019.809.133


Pathomorphological Assessment of Sub-Acute Dermal Toxicity of Morinda citrifolia Linn. Fruit Extract Coated Gold Nano Particles in Wistar Rats
A. R. Pawar1, S. R. Rajurkar1, N. D. Jadhav1*, G. R. Gangane2 and S. Bhalchim2
1Department of Veterinary Pharmacology and Toxicology, India
2Department of Veterinary Pathology, India College of Veterinary and Animal Sciences, Parbhani 431402, India Maharashtra Animal and Fishery Sciences, University, Nagpur, India
*Corresponding author
Abstract:

Gold nanoparticles (GNPs) an emerging part of nanomedicine holds great promise for revolutionizing medical treatments and therapies in areas such as imaging, drug delivery, faster diagnosis, and tissue regeneration, as well as the development of new medical products. But their safety assessment is a major concern. As body system reacts in a different way than isolated cell line than artificial environment, so in the present experiment pathomorphological assessment ofsub-acute dermal toxicity of green synthesized GNPs and coating with Morinda citrifolia fruit extract (McFE) was undertaken. Gold nanoparticles were synthesized by biological reduction of chloroauric acid with Azadirachta indica (neem) 5% leaf extract. The nanoparticles characterized and were confirmed by colour changes from pale yellow to ruby red. These nanoparticles were coated with McFE.For toxicity evaluation.50 Wistar rats of either sex were assigned to five groups. One group was considered healthy, three groups received dermal application of combination of 1 mg/kg GNPs with 150mg/kg McFE (Low dose), 2mg/kg GNPs with 300mg/kg McFE (Medium dose) and 4 mg/kg GNPs with 600mg/kg McFE (High dose) dermal application for 28 days. The last group was considered as satellite group which received 8 mg/kg GNPs with 1200 mg/kg McFE and observed for extra 14 day beyond trial period for checking post withdrawal effect. Grossly mild hemorrhages, congestion and architectural alteration were observed in liver and kidney treated with highest dose. However the histopathological examination of skin revealed no alteration in skin architecture but minimal alterations were observed in liver and kidney where major changes like dilatation of central vein and sinusoidal space, multifocal necrobiotic changes and focal fatty change in liver and cystic degeneration and hyaline cast in lumen of kidney noticed indicating proper absorption and excretion of GNPs from body.


Keywords: Sub-acute dermal toxicity, Morinda citrifolia, Pathomorphology in dermal toxicity, Gold Nano particles.
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How to cite this article:

Pawar, A. R., S. R. Rajurkar, N. D. Jadhav, G. R. Gangane and Bhalchim, S. 2019. Pathomorphological Assessment of Sub-Acute Dermal Toxicity of Morinda citrifolia Linn. Fruit Extract Coated Gold Nano Particles in Wistar Rats.Int.J.Curr.Microbiol.App.Sci. 8(9): 1160-1175. doi: https://doi.org/10.20546/ijcmas.2019.809.133