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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 983-993
DOI: https://doi.org/10.20546/ijcmas.2019.809.116


Assessing the Molecular Diversity in Groundnut (Arachis hypogaea L.) Genotypes Using Microsatellite-Based Markers
Hasanali Nadaf1*, G. Chandrashekhara, B.N. Harish Babu1 and D.L. Savithramma1,2
1Department of Genetics and Plant Breeding, University of Agricultural and Horticultural Sciences Shivamogga, India
2Department of Genetics and Plant Breediing, University of Agricultural Sciences
Bengaluru, India
*Corresponding author
Abstract:

Groundnut (Arachis hypogaea L.) production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level becomes important in all plant breeding programs. The aim of this study was to characterise groundnut genotypes at molecular level using simple sequence repeats (SSR). A total of 30 SSR markers were screened and 20 were found to be polymorphic with an average polymorphic information content (PIC) value of 0.57. Of the 66 groundnut genotypes studied, 57% showed very close relationship (~80% similarity) with one or more genotypes among themselves. The remaining 43% of the groundnut genotypes were distant from each other and could therefore serve as effective parental material for future work. In this study, the SSRs were found to be quite discriminatory in discerning variations between and among groundnut genotypes even where the level of variation was low. Microsatellite based markers therefore represent a useful tool for dissecting genetic variations in most of the cultivated crops, especially in groundnut.


Keywords: Groundnut, DNA extraction, PCR amplification, molecular diversity, SSRs, dendrogram, polymorphic information content, Jaccard’s similarity coefficient
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How to cite this article:

Hasanali Nadaf, G. Chandrashekhara, B.N. Harish Babu and Savithramma, D.L. 2019. Assessing the Molecular Diversity in Groundnut (Arachis hypogaea L.) Genotypes Using Microsatellite-Based Markers.Int.J.Curr.Microbiol.App.Sci. 8(9): 983-993. doi: https://doi.org/10.20546/ijcmas.2019.809.116