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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 422-430
DOI: https://doi.org/10.20546/ijcmas.2019.809.051


Pathology of Avipoxvirus Isolates in Chicken Embryonated Eggs
Bhavesh Sharma1, Nawab Nashiruddullah1*, Jafrin Ara Ahmed2, Sankalp Sharma1 and D. Basheer Ahamad3
1Division of Veterinary Pathology,
2Division of Veterinary Physiology & Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Science and Technology of Jammu, RS Pura-181102, India
3Department of Veterinary Pathology, Veterinary College and Research Institute, Tamil Nadu University of Veterinary and Animal Sciences, Tirunelveli, India
*Corresponding author
Abstract:

Fowlpox virus (FWPV) and Pigeonpox virus (PGPV) isolates from domesticated fowls and pigeons from Jammu region were inoculated on chorioallantoic membrane (CAM) of chicken embryonated eggs (CEE) through three passage levels to observe their cytopathic effects (CPE) and underlying pathology. Development of PGPV induced lesions was earlier than FWPV, and more severe. PGPV progressed from small white opaque lesions from first passage; to CAM thickening, haemorrhages and very prominent and extensive pock lesions at second; and by third passage, focal areas of necrosis was evident. With FWPV, opaque thickening with haemorrhages at first passage; to tiny, discrete, white foci on second; and at third passage, small, round, raised circular white pock were distinctly visible. CAM histology revealed PGPV induced hyperplasia of the chorionic epithelium; oedema, thrombosis, massive fibroblastic proliferation, necrosis and islands of vacuolated epithelial cells containing eosinophilic intra-cytoplasmic inclusions in the mesoderm. FWPV induced lesions were similar but necrosis of the epithelial layers was more widespread; vascular lesions in the mesoderm were more profound, proliferation of fibrous tissue was less, and the absence of inclusion bodies. It was observed that pock morphology on CAM may not be a reliable basis of Avipoxvirus species differentiation, although such variations both grossly and histologically exist.


Keywords: Avipoxvirus, Chicken embryonated eggs (CEE), Chorioallantoic membrane (CAM), Cytopathic effect (CPE), Fowlpoxvirus (FWPV), Pigeonpoxvirus (PGPV)
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How to cite this article:

Bhavesh Sharma, Nawab Nashiruddullah, Jafrin Ara Ahmed, Sankalp Sharma and Basheer Ahamad, D. 2019. Pathology of Avipoxvirus Isolates in Chicken Embryonated Eggs.Int.J.Curr.Microbiol.App.Sci. 8(9): 422-430. doi: https://doi.org/10.20546/ijcmas.2019.809.051