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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 523-531
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.059


Development of Nematophagous Fungi on Dead Adults of Aedes aegypti (Diptera: Culicidae) and Molt Inhibition Experimental 
Fabio Ribeiro Braga1A*, Filippe Elias Freitas Soares2, Gracilene Maria Almeida Muniz Braga3, Juliana Milani Araujo1, Lorendane Millena de Carvalho1, Emy Hiura,
Anderson Rocha Aguiar, Carolina Magri Ferraz, Alessandra Teixeira de Paula1,
Rosana Teixeira Lelis4, Rosane Teixeira Lelis1, Marilvia Dansa de Alencar4,
Rafaela LorenzoniA and Jackson Victor Araújo1*
1Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil
AUniversidade Vila Velha, Vila Velha ES
2Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil
3Escola Superior de Ciências da Santa Casa de Misericordia de Vitoria – EMESCAM
4Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil
*Corresponding author
Abstract:

This study aimed to evaluate development of Duddingtonia flagrans on dead adults of Aedes aegypti (Diptera: Culicidae) and molt inhibition  experimental. The mosquitos used in the experiment were killed previously, and they were used as a basis for chitinase production by the fungus. Two in vitro experimental assays, were performed in separate steps. The assay A, the development of the fungus D. flagrans (AC001) grown from 2% chitin agar on dead adult females of A. aegypti was evaluated. In the assay B, inhibition of the molting of larvae (from second to fourth stage) that were immersed in conidia from AC001 was experimentally evaluated. The development of D. flagrans on adult females of A. aegypti was observed experimentally and it was observed that only the larvae from the control group suffered molting of L2 to adults, with the following percentages: 86% (L4), 6.5% (pupa) and 7.5% (adults). On the other hand, the larvae treated with conidia of AC001 suffered molting until only the larval stage (L4). In this study we observed the development of D. flagrans on dead adults of Aedes aegypti (Diptera: Culicidae) and molt inhibition experimental under laboratory conditions. However, much still needs to be researched before finally being employed as a strategic alternative control.


Keywords: Nematophagous fungi, Chitinase production, Biological control, Fungi.
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How to cite this article:

Fabio Ribeiro Braga, Filippe Elias Freitas Soares, Gracilene Maria   Almeida Muniz Braga, Juliana Milani Araujo, Lorendane Millena de             Carvalho, Emy Hiura, Anderson Rocha Aguiar, Carolina Magri Ferraz,       Alessandra Teixeira de Paula, Rosana Teixeira Lelis, Rosane Teixeira     Lelis, Marilvia Dansa de Alencar, Rafaela Lorenzoni and Jackson Victor Araújo. 2016. Development of Nematophagous Fungi on Dead Adults of Aedes aegypti (Diptera: Culicidae) and Molt Inhibition ExperimentalInt.J.Curr.Microbiol.App.Sci. 5(2): 523-531. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.059