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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2708-2724
DOI: https://doi.org/10.20546/ijcmas.2019.808.313


Effect of Oxytetracycline-dosing on the Growth, Safety and Intestinal Histology of Nile Tilapia, Oreochromis niloticus (L.) Juveniles
R. Beryl Julinta1*, T. Jawahar Abraham1, Anwesha Roy1, Jasmine Singha1, Gadhadar Dash1, Prasenjit Mali1, T. S. Nagesh2, Tapas Kumar Sar3, Prasanna Kumar Patil4 and K. Ashok Kumar5
1Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata-700 094, West Bengal, India
2Department of Fishery Resources Management, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata-700 094, West Bengal, India
3Department of Veterinary Pharmacology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata-700 037, West Bengal, India
4Aquatic Animal Health and Environment Division, ICAR-Central Institute of Brackishwater Aquaculture, Raja Annamalai Puram, Chennai-600 028, Tamil Nadu, India
5Fish Processing Division, ICAR-Central Institute of Fisheries Technology, Willington Island, Cochin-682 029, Kerala, India
*Corresponding author
Abstract:

The present study was carried out to evaluate the effect of oxytetracycline (OTC)-dosing at five different concentrations, viz., 0 mg (0X), 80 mg (1X), 240 mg (3X), 400 mg (5X) and 800 mg (10X) kg-1 biomass day-1 for 30 consecutive days on the growth, safety and intestinal histology of Nile tilapia, Oreochromis niloticus (L.) juveniles. The OTC-residues in the edible muscle were detected at scheduled intervals by LC-MS/MS. A dose-dependent decline in feed intake, biomass and survival were recorded in OTC-dosed fish. The OTC-residue levels were 0, 204.75±45.75, 318.00±0.00, 778.50±145.50 and 684.00±18.00 ng g-1 in 0X, 1X, 3X, 5X and 10X groups, respectively on day 30 OTC-dosing, which reduced subsequently. Relatively mild histopathological lesions including degeneration of epithelial layer, loss of absorptive vacuoles, necrotized intestinal villi, mucinous degeneration, and necrotized absorptive region were observed in the intestine of OTC-dosed fish. Lamina propria swelling was the characteristic change observed in the 10X group on day 15. The observed data revealed that OTC-dosing is reasonably safe at the therapeutic dose of 80 mg kg-1 biomass day-1. However, the precise dose for safe usage of OTC is to be determined according to the culture conditions and species cultured.


Keywords: Oreochromis niloticus, Medicated feed, Oxytetracycline, Biosafety, Histopathology
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How to cite this article:

Beryl Julinta R., T. Jawahar Abraham, Anwesha Roy, Jasmine Singha, Gadhadar Dash, Prasenjit Mali, T. S. Nagesh, Tapas Kumar Sar, Prasanna Kumar Patil and Ashok Kumar K. 2019. Effect of Oxytetracycline-dosing on the Growth, Safety and Intestinal Histology of Nile Tilapia, Oreochromis niloticus (L.) Juveniles.Int.J.Curr.Microbiol.App.Sci. 8(8): 2708-2724. doi: https://doi.org/10.20546/ijcmas.2019.808.313