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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2620-2624
DOI: https://doi.org/10.20546/ijcmas.2019.808.304


Percent Yield of Ovine Mesenchymal Stem Cells (ADMSCs) from Different Sources of Adipose Tissue
R. Gnanadevi1, T. A. Kannan1*, Geetha Ramesh1 and Sabiha Hayath Basha2
1Department of Veterinary Anatomy, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India
2Center for Stem cell Research and Regenerative Medicine, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Adipose derived mesenchymal stem cells are gaining importance due to its wide distribution and ability to differentiate into tissues of mesenchymal origin viz., bone, cartilage, muscle and adipocytes. In the present study, adipose tissue samples from subcutaneous, omental, renal and perineal fat where collected and ADMSCs were isolated using 0.075 percent of collagenase type I. Cell viability was determined by trypan blue exclusion test and the viable cells were counted and cell yield was calculated. In this study, cell yield was found to be highest in renal fat (10.50±0.22 million per ml) and lowest in scrotal fat (0.75±0.09 million per ml). Subcutaneous fat yielded 2.12±0.19 million per ml, perineal fat 2.23±0.08 million per ml and omental fat 1.59±0.18 million per ml. Though yield of ADMSCs was the maximum in renal fat, due to the easy and non-invasive collection procedure and equally high level of viability, subcutaneous fat was considered as the choice of tissue collection site for further studies in sheep.


Keywords: Percent yield, ADMSCs, Stem cell, Sheep
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How to cite this article:

Gnanadevi R., T. A. Kannan, Geetha Ramesh and Sabiha Hayath Basha. 2019. Percent Yield of Ovine Mesenchymal Stem Cells (ADMSCs) from Different Sources of Adipose Tissue.Int.J.Curr.Microbiol.App.Sci. 8(8): 2620-2624. doi: https://doi.org/10.20546/ijcmas.2019.808.304