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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2043-2049
DOI: https://doi.org/10.20546/ijcmas.2019.808.238


Standardization of in vitro Multiplication Protocol for Picrorhiza kurroa (Royle Ex. Benth)
Surjeet Singh Bisht1* and Sanjeev Verma2
1Plant Tissue Culture Laboratory, Department of Molecular Biology and Biotechnology, College of Horticulture, VCSG UUHF Bharsar, Pauri-246123, Uttarakhand, India
2Department of Food Science and Technology, College of Horticulture, VCSG UUHF Bharsar, Pauri-246123, Uttarakhand, India
*Corresponding author
Abstract:

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1. Rooting was initiated with full or half media supplemented with 0.5 to 1.5 mgl-1 of IBA or NAA. In vitro regenerated plantlets were successfully transferred to the soil. Pots having garden soil with organic compost and regular irrigation of half strength MS solution at initial stages are desirable for higher survival of plantlets.


Keywords: Picrorhiza kurroa, Callusing, Shooting, Rooting, Organogenesis
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How to cite this article:

Surjeet Singh Bisht and Sanjeev Verma. 2019. Standardization of in vitro Multiplication Protocol for Picrorhiza kurroa (Royle Ex. Benth).Int.J.Curr.Microbiol.App.Sci. 8(8): 2043-2049. doi: https://doi.org/10.20546/ijcmas.2019.808.238