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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 2485-2490
DOI: https://doi.org/10.20546/ijcmas.2019.808.289


Differential Pathology of Capripox Isolates in Chicken Embryonated Eggs
Sankalp Sharma1*, Nawab Nashiruddullah1, Jafrin Ara Ahmedand Sindhu Berian3
1Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Science and Technology of Jammu, RS Pura-181102, India
2Division of Veterinary Physiology & Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Science and Technology of Jammu, RS Pura-181102, India
3Division of Veterinary Medicine, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e- Kashmir University of Agricultural Science and Technology of Jammu, RS Pura-181102, India
*Corresponding author
Abstract:

During various Capripox outbreaks in Jammu region, either sheep or goats were found to be affected separately by the disease showing the characteristic cutaneous lesions. Even when both sheep and goats were reared together, the disease was restricted to either host only. Clinical scab samples obtained from both sheep and goats were designated as putative Sheep poxvirus (SPPV) and Goatpoxvirus (GTPV). The isolates were processed and inoculated on the chorioallantoic membrane (CAM) of chicken embryonated eggs (CEE) to study the growth characteristics of the isolates. While sheep isolates failed to grow on chorioallantoic membrane (CAM) of chicken embryonated eggs (CEE), goat isolates showed haemorrhages and thickening of membrane with presence of white pock lesions after 2-3 serial passages. Histological examination of the affected CAM showed degeneration of the ectodermal layer together with vacoulation of cells. There were areas of necrosis and presence of eosinophilic intracytoplasmic inclusion bodies within the stromal cells. The putative isolates were confirmed as belonging to SPPV and GTPV genotypes after nucleotide sequencing of partial P32 core protein gene. It is assumed that the differential growth characteristics of the isolates may be an indication of strain variability. Further it is inferred that the non-ability to grow in CEE cannot be used as a criterion either to negate the diagnosis of CaPV, nor the differentiation between SPPV and GTPV.


Keywords: Capripox, Histopathology, Chorioallantoic Membrane (CAM), Chicken Embryonated Eggs (CEE)
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How to cite this article:

Sankalp Sharma, Nawab Nashiruddullah, Jafrin Ara Ahmed and Sindhu Berian. 2019. Differential Pathology of Capripox Isolates in Chicken Embryonated Eggs.Int.J.Curr.Microbiol.App.Sci. 8(8): 2485-2490. doi: https://doi.org/10.20546/ijcmas.2019.808.289