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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 1736-1746
DOI: https://doi.org/10.20546/ijcmas.2019.808.205


Antifungal Activity of Melaleuca Essential Oil Against Lasiodiplodia theobromae in Maize Seeds
Ionaly Gomes de Araújo1, Kevison Romulo da Silva Franca1*, Flavia Mota de Figueredo Alves1, Alda Leaby dos Santos Xavier1, Tiago Silva Lima1, Lídia Pinheiro da Nóbrega1, Antônio Francisco de Mendonça Júnior2, Ana Paula Medeiros dos Santos Rodrigues3 and Tiago Augusto Lima Cardoso4
1Agroindustrial Systems, Federal University of Campina Grande, Pombal, PB, Brazil
2Department of Agronomy, Rural Federal University of Pernambuco, Recife, PE, Brazil
3Agronomy/Plant Protection, Rural Federal University of Semiarid, Mossoró, RN, Brazil
4Phytopathology Laboratory, Federal University of Campina Grande, Pombal, PB, Brazil
*Corresponding author
Abstract:

The fungus Lasiodiplodia theobromae causes black rot in maize seeds, leading to losses in crops production. The fungicides used in the control of black rot are highly toxic, requiring the development of less harmful control techniques. This work evaluates the effect of Melaleuca essential oil against L. theobromae, both under in vitro conditions and on maize seeds. The in vitro experiment compared the growth of the fungus in PDA culture medium containing the oil at 0.25, 0.50, 075, and 1.0%, a negative control, and positive control (Thiram fungicide). The in vivo experiment analyzed the incidence of L. theobromae in maize seeds treated with the essential oil at 0.50, 0.75, and 1.00%, and in the negative and positive controls. After the treatments of seeds, we inoculated them with the fungus and performed sanity tests. In the in vitro test, Melaleuca essential oil inhibited 100% of mycelial growth in the 0.75% concentration. In the in vivo test, the oil at 1.0% concentration reduced the incidence of fungus in the seeds to 45%, being more effective than the synthetic fungicide. Thus, Melaleuca oil has a strong potential as a control agent against fungus L. theobromae in maize seeds.


Keywords: Antimicrobial activity, Mycelial growth, Black rot, Zea mays
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How to cite this article:

Ionaly Gomes de Araújo, Kevison Romulo da Silva Franca, Flavia Mota de Figueredo Alves, Alda Leaby dos Santos Xavier, Tiago Silva Lima, Lídia Pinheiro da Nóbrega, Antônio Francisco de Mendonça Júnior, Ana Paula Medeiros dos Santos Rodrigues and Tiago Augusto Lima Cardoso. 2019. Antifungal Activity of Melaleuca Essential Oil Against Lasiodiplodia theobromae in Maize Seeds.Int.J.Curr.Microbiol.App.Sci. 8(8): 1736-1746. doi: https://doi.org/10.20546/ijcmas.2019.808.205