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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 502-510
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.057


Callus Induction and Plant Regeneration from Leaf Explants of Apple (Pyrus malus L.) cv. Golden Delicious
Ravi Shekhar Kumar*, Charu Joshi and Tapan Kumar Nailwal
Plant Tissue Culture and Molecular Biology Laboratory, Department of Biotechnology, Bhimtal-263136,  Kumaun University, Nainital, Uttrakhand, India
*Corresponding author
Abstract:

The present investigation was conducted to develop a protocol for rapid plant regeneration of apple. The leaf explants of apple (Pyrus malus L.) cultivar viz. Gold Delicious was cultured for plant regeneration. The explants were cultured on MS media supplemented with different concentrations and combinations of BA with IBA and NAA as well as Kinetin with NAA. Maximum induction (80%) of leaf explants of Golden Delicious was observed in medium containing BA + NAA (1.0+0.5 mg/L). MS medium supplemented with different concentrations and combinations of BA, NAA and KIN were also employed for shoot regeneration. MS medium comprises BA (2.0 mg/L) and NAA (0.02 mg/L) in a particular concentration was best for maximum shoot regeneration from leaf calli whereas MS medium containing BA (2.0 mg/L) + NAA (0.02 mg/L) + GA3 (0.4 mg/L) was best for maximum shoot elongation. The micro shoots were successfully rooted on half strength MS medium supplemented with IBA (1.0 mg/L) alone and 96.66% rooted plantlets were obtained using the medium. The in vitro plantlets were acclimatized in jiffy pots after rooting and established in the soil. The protocol of in vitro propagation, presented here is suitable and efficient for cost effective as well as timely production of plantlets for apple (Pyrus malus).


Keywords: Callus induction, Plant regeneration, Apple cultivar, Rooting, Hardening.
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How to cite this article:

Ravi Shekhar Kumar, Charu Joshi and Tapan Kumar Nailwal. 2016. Callus Induction and Plant Regeneration from Leaf Explants of Apple (Pyrus malus L.) cv. Golden DeliciousInt.J.Curr.Microbiol.App.Sci. 5(2): 502-510. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.057