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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 77-81
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.010


Rapid Detection of Mycobacteria using automated Detection/ Incubation System in Exudative Pleural Effusion in Alexandria Main University Hospital
Ahmed Youssef Shaaban1 and Nesrine Fathi Hanafi2*
1Chest Department,
2Medical Microbiology and Immunology,Faculty of Medicine, Alexandria University, Egypt
*Corresponding author
Abstract:

Studies have been targeting to evaluate newer automated culture systems techniques for different types of samples to provide a more rapid diagnosis relying on sensitive and specific principle with the ability to discriminate between viable and dead mycobacterial bacilli. This study aimed at assessment of bactalert, biomerieux ® culture system as a rapid diagnostic tool for tuberculous pleural effusion fluid in comparison with Lowenstein Jensen conventional culture and zeil nelseen stained smears. Pleural aspirate collected from 60 patients presented with exudative pleural effusion was centrifuges and the deposit divided to be inoculated on bactalert bottles, on Lowenstein Jensen media and the third part was fixed on a slide and stained with zeil nelseen stain. Our study confirmed previous reports that bact Alert system has very high sensitivity still with lower specificity. This finding might consider Bact Alert system a reliable, easy, rapid screening test for tuberculous pleurisy that needs confirmation via other laboratory assays.


Keywords: Tuberculosis,Tuberculous pleural effusion fluid,Mycobacterial bacilli.
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How to cite this article:

Ahmed Youssef Shaaban and Nesrine Fathi Hanafi. 2017. Rapid Detection of Mycobacteria using automated Detection/ Incubation System in Exudative Pleural Effusion in Alexandria Main University HospitalInt.J.Curr.Microbiol.App.Sci. 6(2): 77-81. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.010