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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 2075-2080
DOI: https://doi.org/10.20546/ijcmas.2019.807.249


Biofilm Detection amongst Metallo Beta Lactamase (MBL) Producing Clinical Isolates of Pseudomonas aeruginosa
Hardik K. Bhavsar1, Roma Goyal2* and Anil Kumar2
1Department of Microbiology, GCS Medical College, Hospital and Research Centre, Ahmedabad, Gujarat, India
2Department of Microbiology, MLB Medical College and Hospital, Jhansi, Uttar Pradesh, India
*Corresponding author
Abstract:

In recent decades the global emergence the development of multidrug-resistant and pan drug resistance in Pseudomonas aeruginosa has been observed. This opportunistic pathogen is responsible for causing various infections especially in the intensive care units, resulting for almost 10% of nosocomial infections. The resistance to Metallo beta-lactamase in P. aeruginosa strains has been related with bacterial biofilm formation. So, the aim of this study was to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa. A total of 200 P. aeruginosa isolates were isolated from different clinical specimens were included in this study. Modified Kirby-Bauer disc diffusion technique was used for antimicrobial susceptibility testing. MBL production was detected by E-Test, while microtiter plate assay was used for detection of biofilm production. Out of 200 isolates, 21 isolates were MBL producing whereas, 32 were able to produce biofilm as shown microtiter plate assay. The highest tendency of biofilm production was shown by Pseudomonas aeruginosa isolated from urine followed endotracheal tube. Highest rates of susceptibility of P.aeruginosa were seen toward colistin. In our study, biofilm production and metallo beta-lactamase production were observed among Pseudomonas aeruginosa. However, no statistically significant association could be established between biofilm production and metallo beta-lactamase production.


Keywords: Pseudomonas aeruginosa, Clinical isolates, Antibiotic resistance, Biofilm, MBL
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How to cite this article:

Hardik K. Bhavsar, Roma Goyal and Anil Kumar. 2019. Biofilm Detection amongst Metallo Beta Lactamase (MBL) Producing Clinical Isolates of Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 8(7): 2075-2080. doi: https://doi.org/10.20546/ijcmas.2019.807.249