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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 2043-2050
DOI: https://doi.org/10.20546/ijcmas.2019.807.245


Isolation and Characterization of Yeast from Tomato Plants for Biological Control of Alternaria solani
G. Ashiwini* and B.C. Mallesha
Department of Agricultural Microbiology, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka (India)
*Corresponding author
Abstract:

Nine different yeast isolates from various parts of tomato plant were isolated from two different locations of Bengaluru. The population on tomato leaves varied from 2.01-3.5 x104 cfu/cm², on stems from 6.4-7.31 x104 cfu/g fresh weight and on fruits from 6.7-7.38 x104 cfu/g fresh weight. The yeast isolates were characterized for utilization of different carbon sources. Out of nine isolates, four yeast isolates TPL-I, RTPL, RTPS- II and RTPF- II produced lipase enzyme. Further, they were evaluated for biocontrol activity against Alternaria solani in in vitro conditions. Among the yeast isolates, TPL-I yeast isolate showed strongly positive result by forming maximum zone of inhibition (9mm), produced minimum dry mycelial weight (40.07 mg) of pathogen and also showed highest percentage of inhibition of conidial germination (80.50 %) compared to other yeast isolates. Four yeast isolates produced relatively higher concentrations of IAA ranging from 0.21-0.68 μg/ml and the maximum concentration of IAA was produced by TPL- I.


Keywords: Yeasts, Tomato, Alternaria solani and Biocontrol activity
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How to cite this article:

Ashiwini, G. and Mallesha, B.C. 2019. Isolation and Characterization of Yeast from Tomato Plants for Biological Control of Alternaria solani.Int.J.Curr.Microbiol.App.Sci. 8(7): 2043-2050. doi: https://doi.org/10.20546/ijcmas.2019.807.245