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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 2030-2033
DOI: https://doi.org/10.20546/ijcmas.2019.807.243


Study on Cytotoxicity and Virus Titration of Chitosan Nanoparticles Encapsulated Live PPR Virus Vaccine
J. Akalya*
Cattle Breeding and Fodder Development, Perambalur, Tamilnadu, India
*Corresponding author
Abstract:

The cytotoxicity and virus titration of chitosan nanoparticles encapsulated live PPR virus vaccine (PPRV-CS-NPs) was analyzed in this study. Live attenuated PPR virus vaccine (AR-87) was encapsulated in chitosan nanoparticles using ionic gelation technique. The PPRV-CS-NPs were tested for cytotoxicity study in Vero cells with different concentrations (1-40g).The cell viability was more than 80 per cent with all the concentration of the PPRV-CS-NPs tested. This indicates that the PPRV-CS-NPs are not toxic even in concentration higher than the required vaccine dose. The viability of PPRV (AR-87) in the PPRV-CS-NPs were tested by releasing the virus from nanoparticles by trypsin digestion. The virus titration was carried out in Vero cells and the titre was found to be 10 4.6TCID50/ml indicating that chitosan encapsulation was able to maintain virus infectivity.


Keywords: Chitosan, PPRV, Vero cells, Cytotoxicity, Titration
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How to cite this article:

Akalya, J. 2019. Study on Cytotoxicity and Virus Titration of Chitosan Nanoparticles Encapsulated Live PPR Virus Vaccine.Int.J.Curr.Microbiol.App.Sci. 8(7): 2030-2033. doi: https://doi.org/10.20546/ijcmas.2019.807.243