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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 1835-1843
DOI: https://doi.org/10.20546/ijcmas.2019.807.218


A Comparative Evaluation for Detection of Hepatitis B Surface Antigen and Anti HCV Antibodies by Rapid Diagnostic Tests and ELISA Method at Govt. Medical College, Dungarpur, India
Bhupendra Kumar Mandawat* and Vipul Mathur
Department of Microbiology, Government Medical College, Dungarpur (Rajasthan), India
*Corresponding author
Abstract:

Hepatitis B virus and hepatitis C virus share common modes of transmission i.e. by blood and blood products mainly and also noticed in drug addicts. Hepatitis B virus and Hepatitis C virus have been recognized as a major pathogen causing significant morbidity and mortality throughout the world including India. The most important markers for HBV and HCV infection are HBsAg and Anti HCV antibodies respectively. Detection of infection markers for these agents is a major challenge in a resource poor setting.For proper diagnosis of infection as well as disease management and prevention, identification of appropriate test kit is necessary. The aims of this study were to compare Rapid tests with ELISA for Hepatitis B and Hepatitis C virus infection. The ultimate goal of this study was to recommend most reliable and cost-effective rapid card test for the diagnosis of HBV and HCV in areas where advance diagnostic facilities are not available. The present study was conducted in the clinical microbiology laboratory of Govt, Medical College, Dungarpur from period of 1st March, 2019 to 15th May2019 to comparative evaluate the detection of HBsAg and Anti HCV antibodies by rapid diagnostic tests and ELISA method. In our study, total no. of 1756samples were tested for HBsAg and 188 samples for HCV by ELISA method (gold standard) as a confirmatory methodand samples found reactive were again tested by rapid test kits. On testing for 1756 samples of HBsAg, 78 samples were reactive by ELISA but only 70 were reactive in Rapid tests and Out of 188 samples for HCV, 7 were reactive for Anti HCV antibodies by ELISA but only6 samples were reactive by Rapid tests. For proper diagnosis of infection as well as disease management and prevention, identification of appropriate test kit is necessary. The card test’s sensitivity and specificity is comparable with ELISA. These rapid kits are cheaper and easy to perform and their use should be encouraged at rural settings. ELISA is much more sensitive than rapid tests for screening of infections like HBsAg and HCV.


Keywords: HBV, HCV, HBsAg, Anti HCV antibodies, Rapid test, ELISA
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How to cite this article:

Bhupendra Kumar Mandawat and Vipul Mathur. 2019. A Comparative Evaluation for Detection of Hepatitis B Surface Antigen and Anti HCV Antibodies by Rapid Diagnostic Tests and ELISA Method at Govt. Medical College, Dungarpur, India.Int.J.Curr.Microbiol.App.Sci. 8(7): 1835-1843. doi: https://doi.org/10.20546/ijcmas.2019.807.218