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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 152-163
DOI: https://doi.org/10.20546/ijcmas.2019.807.019


Isolation and Identification of PPFM Bacterial Isolates Isolated From Direct Seeded Rice Growing Areas of Hyderabad-Karnataka
J. Raghavendra1*, G.P. Santhosh1, Mahadevaswamy1, S. Shubha1 and M.V. Ravi2
1Department of Agriculture Microbiology,
2Department of Soil Science and Agricultural Chemistry, University of Agriculture Sciences, Raichur-585104, Karnataka, India
*Corresponding author
Abstract:

A total of 50 PPFM’s were isolated from rhizosphere soil and phyllosphere of direct seeded rice regions of Hyderabad-Karnataka. Samples of leaves and rhizosphere soils were collected from direct seeded rice grown in different location (Raichur district, Bellary district, Koppal district and Yadgir district). Thereafter, all the classical recommended methods used for identification of all the obtained isolates were carried out including, morphological, microscopical as well as biochemical characteristics. The results revealed that all the isolates were gram negative, rod shaped, motile. Colonies were pink, pale pink or dark pink in color due to pigmentation. Biochemical characteristics revealed that all the isolates including reference strain confirmed to be negative for Methyl red and Voges-Proskauer test while, positive for oxidase, urease, catalase activity, indole production and citrate utilization. None of the isolates could reduce nitrate to nitrite, whereas; the hydrolysis of casein and starch was not recorded in any of the isolates tested They can be cultivated on ammonium mineral salt (AMS) agar with methanol colonies were pink, pale pink or dark pink in color due to pigmentation and variations in colonial morphology. Based on the observed characteristics, the isolates obtained belong to the genus Methylobacterium.


Keywords: PPFM’s, Direct seeded rice, Rhizosphere, Phyllosphere and Methylobacterium
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How to cite this article:

Raghavendra, J., G.P. Santhosh, Mahadevaswamy, S. Shubha and Ravi, M.V. 2019. Isolation and Identification of PPFM Bacterial Isolates Isolated From Direct Seeded Rice Growing Areas of Hyderabad-Karnataka.Int.J.Curr.Microbiol.App.Sci. 8(7): 152-163. doi: https://doi.org/10.20546/ijcmas.2019.807.019