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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 707-720
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.085


Phytochemical Screening and In vitro Antimicrobial Properties of Crude Leaf Extracts of Wrightia tinctoria R.Br.
Sravanthi Maddila and K.P.J. Hemalatha*
Department of Biochemistry, Andhra University, Visakhapatnam, 530003, Andhra Pradesh, India
*Corresponding author
Abstract:

The crude hexane, ethyl acetate and aqueous leaf extracts of medicinal plant Wrightia tinctoria R.Br. were evaluated for in vitro antimicrobial activity against selected bacterial and fungal strains. Preliminary phytochemical screening of the three crude leaf extracts revealed the presence of phenols, alkaloids, tannins, flavonoids, terpenoids and sterols. The extracts were screened against three Gram positive strains (Bacillus subtilis, Bacillus licheniformis and Streptococcus pneumoniae), two Gram negative strains (Escherichia coli and Sphingomonas sanguinis) and against three fungal strains (Aspergillus flavus, Aspergillus niger and Candida albicans). Antimicrobial activity of these crude extracts was determined using Agar well diffusion method. Agar broth and Potato dextrose broth dilution method was used to determine the minimum inhibitory concentration. Ampicillin and Flucanazole were used as standards for bacteria and fungi respectively. The results of in vitro antimicrobial activity showed that hexane, ethyl acetate and aqueous crude leaf extracts were active on all microbial strains tested at different concentrations, with hexane leaf extract exhibiting more activity. Results obtained from the present study showed that the leaf extracts of Wrightia tinctoria R.Br. have considerable antimicrobial activity and hence, they may be used for further extraction and isolation of antimicrobial compounds which may find a place in medicine industry as constituents of antibiotics.
 


Keywords: Wrightia tinctoria, phytochemical screening, antimicrobial activity, minimum inhibitory concentration (MIC).
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How to cite this article:

Sravanthi Maddila and Hemalatha, K.P.J. 2017. Phytochemical Screening and In vitro Antimicrobial Properties of Crude Leaf Extracts of Wrightia tinctoria R.Br.Int.J.Curr.Microbiol.App.Sci. 6(1): 707-720. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.085