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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 464-474
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.052


A study of Bioremediation of Methyl Parathion in vitro using Potential Pseudomonas sp. isolated  from Agricultural Soil, Visakhapatnam, India
S.Sharmila Begum1* and A.Arundhati2
1Department of Biotechnology, Dr. L.Bullayya PG.College, Visakhapatnam, Andhra Pradesh, India
2Department of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India
*Corresponding author
Abstract:

The present investigation deals with screening and isolation of Methyl parathion (MP) degrading bacteria from local agriculture soil  and study of its biodetoxification efficiency in vitro using potential Pseudomonas sp R2.  The potent  isolates that could degrade Methyl Parathion were identified as Pseudomonas species R1, R2 and R3 respectively. Effect of pH and temperature on growth profile of these isolate revealed 30˚C as the optimum temperature at 500μg/ml. But, the   growth was optimized at pH 7 and 37˚C to be used for an added advantage in cloning the  Methyl Parathion degrading (mpd) in E. coli.  The  three Pseudomonas isolates were found to tolerate a concentration of 3800µg/ml and R2 isolate identified as Pseudomonas aeruginosa species  which is capable of  degrading 1920µg/ml MP after 48 hrs in  in vitro studies.


Keywords: P. aeruginosa,  Methyl Parathion, Pesticide, Biodetoxification
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How to cite this article:

Sharmila Begum, S. and  Arundhati, A. 2016. A study of Bioremediation of Methyl Parathion in vitro using Potential Pseudomonas sp. isolated  from Agricultural Soil, Visakhapatnam, IndiaInt.J.Curr.Microbiol.App.Sci. 5(2): 464-474. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.052