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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2913-2919
DOI: https://doi.org/10.20546/ijcmas.2019.806.350


Phylogenetic Relationship of Venturia carpophila, the Causal Agent of Almond Scab from Kashmir Valley as Inferred by ITS nr DNA
M.S. Dar1, Mushtaq Ahmad2, M.D. Shah3, Nazir A. Bhat4, M.Anwar Khan5, and Bilal A. Padder3
1Division of Plant Pathology, Faculty of Agriculture, Wadura Sopore, SKUAST-Kashmir 191121, India
2Directorate of Extension, SKUAST-Kashmir, Shalimar, Srinagar, 190025, India
3Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, SKUAST-Kashmir, Shalimar Srinagar 190025, India
4Mountain Field Crop Research Centre, Khudwani, Anantnag, 192001, India
5Division of Genetics and Plant Breeding, Faculty of Agriculture, Wadura Sopore, SKUAST-Kashmir 191121, India
*Corresponding author
Abstract:

The fungus Venturia carpophila causes scab in almond. In order to gain insight, an in vitro culture of the fungus has been established and its identity confirmed by its nr DNA. Internal transcribed spacer-ribosomal DNA (ITS-rDNA); the fungal molecular marker was used for molecular analysis. The target region of rDNA (ITS1-5.8S-ITS2) of this species was amplified using universal fungal primers (ITS1 and ITS4). The sequencing of amplified products and their subsequent Basic Local Alignment Search Tool analysis confirmed the identification of species by comparing the sequence of the species with respective species sequences present in Gen Bank. Phylogenetic analysis also confirmed the identification of fungi belongs to Venturiaceae family having 100% similarity to other V. carpophila species.


Keywords: Almond scab, nr DNA, Internal transcribed spacer, Phylogenetics, Venturia carpophila
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How to cite this article:

Dar, M.S., Mushtaq Ahmad, M.D. Shah, Nazir A. Bhat, M.Anwar Khan and Bilal A. Padder. 2019. Phylogenetic Relationship of Venturia carpophila, the Causal Agent of Almond Scab from Kashmir Valley as Inferred by ITS nr DNA.Int.J.Curr.Microbiol.App.Sci. 8(6): 2913-2919. doi: https://doi.org/10.20546/ijcmas.2019.806.350