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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2310-2320
DOI: https://doi.org/10.20546/ijcmas.2019.806.274


SSR Marker-Based Molecular Characterization of Some Upland Taro (Colocasia esculenta L. Schott) Cultivars of North-East India
Khatemenla1*, S. Alam1, Madhumita Barooah2, Deepa B. Phookan1, Prakash Kalita3, Madhumita C. Talukdar1 and Star Luikham4
1Department of Horticulture,
3Department of Crop Physiology,
4Department of Plant Pathology, Assam Agricultural University, Jorhat-785013, India
2Department of Agricultural Biotechnology, Assam Agricultural University,
Jorhat-785013, India
*Corresponding author
Abstract:

Ten SSR markers were used to characterize 22 taro cultivars from North East India. The study revealed that the SSR primers like uq201- 302, Ce1 B03 and Ce1 C06 with PIC=0.82, 0.76 and 0.76 respectively were found to be the most informative markers which can be used for future molecular works on taro. The Jaccard’s similarity coefficient analysis ranging from 0.103 to 0.89 presented high level of genetic distance as no true duplicates were detected. The dendrogram constructed categorized the cultivars into two main clusters I and II. Only one cultivar, Damor Dema collected from Assam clustered on its own in cluster I and the second cluster II contained the remaining 21 cultivars. It was also observed that geographical origin of the cultivars did not bear any relationship with the molecular classification. The study revealed that cross between genetically distantly related cultivars like Damor Dema and Garo Kochu; Sree Kiran and Makhuti; and Damor Dema and Bor Kochu are advisable. However, crosses between genetically closely related cultivars like Boga Ahina and Red Garo, will have to be avoided for future breeding programme. Characterization of taro cultivars using SSR markers would contribute to the knowledge of genetic relationships between different cultivars.


Keywords: Taro, cultivars, SSR, molecular and markers
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How to cite this article:

Khatemenla, S. Alam, Madhumita Barooah, Deepa B. Phookan, Prakash Kalita, Madhumita C. Talukdar and Star Luikham. 2019. SSR Marker-Based Molecular Characterization of Some Upland Taro (Colocasia esculenta L. Schott) Cultivars of North-East India.Int.J.Curr.Microbiol.App.Sci. 8(6): 2310-2320. doi: https://doi.org/10.20546/ijcmas.2019.806.274