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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2125-2134
DOI: https://doi.org/10.20546/ijcmas.2019.806.252


Evaluation of Genetic Diversity in Gloriosa superba L, an Endangered Medicinal Plant Using Molecular Marker
K.S. Sahana1*, R. Gnanam1, S. Rajesh1 and K. Rajamani2
1Department of Plant Biotechnology, Tamil Nadu Agriculture University, Coimbatore-641003, Tamil Nadu, India
2Department of Medicinal and Aromatic Crops, Tamil Nadu Agriculture University, Coimbatore-641003, Tamil Nadu, India
*Corresponding author
Abstract:

Genetic diversity evaluation among 16 Gloriosa accessions collected from different locations in Tamil Nadu was studied by using Inter-simple sequence repeat (ISSR) markers. Thirty six ISSR primers were used, among that 16 primers showed 83.56 per cent polymorphism and produced 213 amplicons. This indicates that there is a high level of variation at the genetic level among these accessions. The primer UBC-807 showed the highest PIC value (0.958), which represented the high efficiency of the individual primer. Gloriosa accessions GSU-6 and GSU-7 are highly similar with 68 per cent and GSU-7 and GSU-16 are distinctly similar with 39 per cent. Cluster analysis was done based on similarity coefficient value obtained from banding pattern of ISSR marker. The 16 Gloriosa accessions were grouped into two major clusters, of which one cluster contained all 15 accessions whereas as another cluster contained GSU-16 accession alone. This represents that the Gloriosa accession GSU-16 is genetically distinct from other accessions based on banding pattern.


Keywords: Genetic diversity, Gloriosa, Inter-simple sequence repeat (ISSR), Cluster analysis
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How to cite this article:

Sahana, K.S., R. Gnanam, S. Rajesh and Rajamani, K. 2019. Evaluation of Genetic Diversity in Gloriosa superba L, an Endangered Medicinal Plant Using Molecular Marker.Int.J.Curr.Microbiol.App.Sci. 8(6): 2125-2134. doi: https://doi.org/10.20546/ijcmas.2019.806.252