Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1332-1342
DOI: https://doi.org/10.20546/ijcmas.2019.806.161


Molecular and Biochemical Identification of Reactive Red-11 Decolorising Klebsiella oxytoca CMGS-3 Bacterial Strain and Study of Toxic Effect on Plants
Madhuri R. Basutkar, Channappa T. Shivannavar* and Subhaschandra M. Gaddad
Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi - 585106, Karnataka, India
*Corresponding author
Abstract:

The tentatively identified bacterial strain was showed decolorisation of 82.3% in mineral salt of medium with 100mg/L Reactive red-11 without any external nutritional source within 72 hours. Finally optimization done at pH-8, temperature -35ºC and with 0.1 % (1gm/L) yeast extract and dye concentration was 200mg/l and decolorisation confirmed around of 94% within 16 hours of incubation. Further work carried on The identification of bacterial isolate and identified as Klebsiella oxytoca CMGS-3 by16s rRNA technology (Gene bank accession num- KT602858), and various biochemical and sugar utilization tests were done, finally complete decolorisation confirmed by the FT-IR analysis, and green house studies were done to check the toxic effect of dye; here the decolorized and untreated dye used to study toxic effect on crops which were widely grown agriculture crops and CMGS-3 Strain showed better results in germination as well as plant growth. Through these results, it concludes isolate CMGS-3 was the better candidate for the decolorisation of wide range of reactive dyes and it is preserved in IMTECH, (Chandigarh) with the MTCC num-12532 for the usage of waste water treatment.


Keywords: Decolorisation, accession number, Klebsiella oxytoca, germination, IMTECH, MTCC
Download this article as Download

How to cite this article:

Madhuri R. Basutkar, Channappa T. Shivannavar and Subhaschandra M. Gaddad. 2019. Molecular and Biochemical Identification of Reactive Red-11 Decolorising Klebsiella oxytoca CMGS-3 Bacterial Strain and Study of Toxic Effect on Plants.Int.J.Curr.Microbiol.App.Sci. 8(6): 1332-1342. doi: https://doi.org/10.20546/ijcmas.2019.806.161