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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 588-601
DOI: https://doi.org/10.20546/ijcmas.2019.806.069


An Evaluation Study of Different White Rot Fungi for Degradation of Pine Needles under Solid State Fermentation
Nivedita Sharma*, Nisha Sharma and Dimple
Microbiology Research laboratory, Department of Basic sciences, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni (Solan) Himachal Pradesh 173230, India
*Corresponding author
Abstract:

The present study was focused on the biodegradation of pine needles by the production of hydrolytic enzymes viz. cellulase, xylanase, laccase as well as reducing sugars production w.r.t optimization of moisture (Basal salt media) ratio for the newly isolated white rot fungi Phanerochaete sp. S7, Armillaria sp. K3 and Phanerochaete chrysosporium [NCIM 1197] were optimized under solid state fermentation and maximum of cellulase (52.27 U/g) with specific activity of 2.10 was observed in P. chrysosporium using untreated pine needles as substrate at 1:4 of moisture ratio, while maximum of xylanase and laccase were expressed by white rot Armillaria sp K3 at 1:4 of moistening agent i.e.174.00 U/g and 5.94 U/g with respect to their specific activities of 7.65 and 0.28 using untreated pine needles. In case of reducing sugars maximum of 15.48 mg/g was released at 1:4 of moisture using pretreated pine needles which is followed by the value of 14.88 mg/g at 1:3 by P. chrysosporium in untreated pine needles. In our study the newly isolated white rot fungi Phanerochaete sp. S7 and Armillaria sp. K3 were found to be potential strains for the hyper production of lignin degrading enzyme i.e. laccase as compared to the standard strain Phanerochaete chrysosporium [NCIM 1197].


Keywords: Cellulase, Xylanase, Laccase, Solid state fermentation, Pine needles
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How to cite this article:

Nivedita Sharma, Nisha Sharma and Dimple. 2019. An Evaluation Study of Different White Rot Fungi for Degradation of Pine Needles under Solid State Fermentation.Int.J.Curr.Microbiol.App.Sci. 8(6): 588-601. doi: https://doi.org/10.20546/ijcmas.2019.806.069