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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 411-415
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.049


A  Study by Double Disc Diffusion (DDDT) Method to Compare Ceftazidime + Clavulanic Acid and Cefotaxime + Clavulanic Acid for Detection of Extended Spectrum Β- Lactamase among Escherichia coli and Klebsiella pneumoniaee in Urinary Isolates
R. Sujatha*, Anil Kumar and Vikas Mishra
 Department of Microbiology, Rama Medical College, Kanpur, India
*Corresponding author
Abstract:

The detection of extended- spectrum β-lactamase (ESBL) producing bacteria is important for infection control and epidemiological surveillance. The purpose of the present study was to compare two phenotypic methods for detection of ESBL positive Escherichia coli and Klebsiella pneumoniae in urinary isolates. This study was carried out in the Department of Microbiology, Rama Medical College, Kanpur(U.P), India from November 2015 to August 2016. Patients of all ages and genders were taken as study population. Lactose fermenting gram negative bacilli(GNB) were analyzed and routine susceptibility testing was performed. Screening test for ESBL production was done by two disc diffusion methods Ceftazidime and Ceftazidime+Clavulinic acid(CAZ/CAZC) and Cefotaxime and Cefotaxime+ Clavulinic acid (CTX/CTXC). In our study, 576 urine samples have been collected, from them 369 uropathogens were isolated 102 (17.07%) Escherichia coli, 66 (11.45%) Klebsiella pneumoniae and 201 others(34.89%) (including Staphylococcus aureus, CONS, Proteus species, Pseudomonas aeruginosa, Acinetobacter species and candida species) respectively. The prevalence of ESBL producing Escherichia coli and Klebsiella pneumoniae in our study is 30.39% and 36.36% respectively. Of the 65 Escherichia coli and 41 Klebsiella pneumoniae Screned For ESBL . 46 isolate were detected by combined disc method. The use of ceftazidime(30 µg) and ceftazidime + clavulanic acid(30µg/ 10µg) detected 46 isolates as ESBL positive as compared to cefotaxime(30 µg)  and cefotaxime + clavulanic acid(30µg/ 10µg) which detected 33 isolates as ESBL positive. The use of ceftazidime(30 µg) and ceftazidime + clavulanic acid(30µg/ 10µg) DDDT phenotypic methods are useful diagnostic tool for detection of ESBL in gram negative bacilli..


Keywords: Extended-spectrum β-lactamase, Phenotypic detection, double disc diffusion test.
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How to cite this article:

Sujatha, R., Anil Kumar, Vikas Mishra. 2017. A  Study by Double Disc Diffusion (DDDT) Method to Compare Ceftazidime + Clavulanic Acid and Cefotaxime + Clavulanic Acid for Detection of Extended Spectrum Β- Lactamase among Escherichia coli and Klebsiella pneumoniaee in Urinary Isolates.Int.J.Curr.Microbiol.App.Sci. 6(1): 411-415. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.049