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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 231-240
DOI: https://doi.org/10.20546/ijcmas.2019.806.026


In-vivo Assay of Escherichia coli Microorganisms in a Live Organ using Voltammetric Microprobe
Suw Young Ly*, Chaeyun Lee,Yun Ji Kim, Min Ji won, Sih Yun Jun, Yun June Hwang,Seung Ki Kim, Seung Jun Lee, Kyung Lee
Biosensor Research Institute, Seoul National University of Science and Technology 172 Gongreung 2 dong, Nowon gu, Seoul, South Korea 139-743
*Corresponding author
Abstract:

The presence of Escherichia coli (EC) microorganisms in live organs can cause foodborne illnesses and food-related deaths. Here, EC assay was performed using a microcopper three-electrode (MCE) system, where a handmade MCE was used as a working electrode and Ag/AgCl as reference and platinum counter electrodes. Under a 1.0-ml EC standard, the diagnostic optimum conditions were sought. The analytical oxidation potential was obtained at -0.2 V via positive scan. Under these conditions, the stripping linear working range was attained with 0.2-0.7 mg/mL EC variations. A statistic relative standard deviation of 6.78% (n=13) was obtained by 1.0 mg/mL EC using 0.0 sec accumulation time. Under optimum conditions, the detection limit was 0.6 mg/mL. Here, the diagnostics were explored real-time in the blood vascular system of a live frog. Moreover, which probe can be used for in-vivo clinical application in animal organs (heart, colon, lungs, and gallbladder) was determined as the patient’s peak current increased a hundred times more than in the negative tissue. The sensing time was only 30 sec. This method is simpler than the common PCR amplification, electrophoresis, and photometric detection methods and can be useable for fluorescence analytical catheter probe.


Keywords: Real time detection, Escherichia coli, Microorganisms, In vivo organ, Microprobe
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How to cite this article:

Suw Young Ly. 2019. In-vivo Assay of Escherichia coli Microorganisms in a Live Organ using Voltammetric Microprobe.Int.J.Curr.Microbiol.App.Sci. 8(6): 231-240. doi: https://doi.org/10.20546/ijcmas.2019.806.026