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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1581-1589
DOI: https://doi.org/10.20546/ijcmas.2019.805.183


Allele Specific PCR Based Genotyping Reveals Lack of Bovine Leukocyte Adhesion Deficiency, Bovine Citrullinaemia and Complex Vertebral Malformation Carriers in Indian Cattle Population
Gebremedhin Gebreselassie1*, Sachinandan De2, Ayan Mukherjee2 and Moloya Gohain2
1College of Dryland Agriculture, Jigjiga University, Jigjiga, Ethiopia
2Animal Biotechnology Center, National Dairy Research Institute, Karnal-132001, Haryana, India
*Corresponding author
Abstract:

Bovine Leukocyte Adhesion Deficiency (BLAD), Bovine Citrullinaemia (BC), and Complex Vertebral Malformation (CVM) are the common autosomal recessive disorders in cattle affecting dairy industry. The present study was designed to standardize allele-specific PCR based genotyping method for examining the occurrence of these disorders in Indian cattle population. A total of 327 indigenous (Bos indicus), exotic (HF) cattle breeds and HF crossbreds (Bos taurus × Bos indicus) from various parts of India were screened. An allele specific PCR technique was applied to detect mutation in specific DNA target sequences. The amplified products were purified and sequenced to confirm the change in nucleotide in the selected regions. Allele-specific PCR-based screening methodology was optimized for genotyping. No animals were found as carrier of these genetic abnormalities in the present study. Allele-specific PCR-based screening methodology may be efficiently employed to screen the animal population for these genetic defects. All tested animals were free from the aforementioned genetic disorders. But this may not provide us conclusively cattle herds maintained at the study area are free from these genetic disorders. Therefore, it is highly recommended to continue screening procedure to check undesirable transmission mutated version of alleles in Indian cattle population.


Keywords: Genetic defects, Cattle, Genotyping, Genetic disorder, India
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How to cite this article:

Gebremedhin Gebreselassie, Sachinandan De, Ayan Mukherjee and Moloya Gohain. 2019. Allele Specific PCR Based Genotyping Reveals Lack of Bovine Leukocyte Adhesion Deficiency, Bovine Citrullinaemia and Complex Vertebral Malformation Carriers in Indian Cattle Population.Int.J.Curr.Microbiol.App.Sci. 8(5): 1581-1589. doi: https://doi.org/10.20546/ijcmas.2019.805.183