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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1426-1436
DOI: https://doi.org/10.20546/ijcmas.2019.805.163


Prevalence and Molecular Detection of Blood Protozoa in Domestic Pigeon
Munmi Saikia1*, Kanta Bhattacharjee1, Prabhat Chandra Sarmah1, Dilip Kr. Deka1, Shantanu Tamuly2, Parikshit Kakati1 and Pranab Konch3
1Department of Parasitology
2Department of Biochemistry
3Department of Pathology,
College of Veterinary Science, Khanapara, Guwahati-781022, Assam, India
*Corresponding author
Abstract:

The present study was carried out to know the status of haemoprotozoan infection of domestic pigeon in Assam by microscopic examination of blood of pigeons for a period of one year which revealed an overall prevalence of 53.39%. Three species viz. Haemoproteus columbae (29.93%), Plasmodium relictum (21.29%) and Leucocytozoon sp. (2.16%) were identified either in single or mixed infection. According to age, highest prevalence was recorded in adult (61.81%) and lowest in squab (36.25%). Comparatively, infection was recorded higher in females (58.22%) than males (48.79%). Season wise, infection was recorded highest during Pre-monsoon (72.22%) and lowest during Post-monsoon. Amplification of cyt b gene of Haemoproteus columbae in positive samples by PCR showed clear band at 207 bp. Amplification of mt- cyt b gene of Haemoproteus spp. and Plasmodium spp. by PCR on positive samples revealed clear band at 525 bp.


Keywords: Pigeon, Haemoprotozoa, Prevalence, PCR, Assam
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How to cite this article:

Munmi Saikia, Kanta Bhattacharjee, Prabhat Chandra Sarmah, Dilip Kr. Deka, Shantanu Tamuly, Parikshit Kakati and Pranab Konch. 2019. Prevalence and Molecular Detection of Blood Protozoa in Domestic Pigeon.Int.J.Curr.Microbiol.App.Sci. 8(5): 1426-1436. doi: https://doi.org/10.20546/ijcmas.2019.805.163