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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1283-1288
DOI: https://doi.org/10.20546/ijcmas.2019.805.146


Detection of Mycobacterium tuberculosis in Dog of Assam
Acheenta G. Barua1*, Koushik Kakoty1, Pranjal M. Nath1 and Bhaben Baishya2
1Department of Veterinary Public Health,
2Teaching Veterinary Clinical Complex, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, India
*Corresponding author
Abstract:

A total of 52 suspected dogs with symptoms of harsh, chronic non-productive coughing, fever and anorexia were examined for tuberculosis in the Teaching Veterinary Clinical Complex, Guwahati, Assam. SIT carried out by injecting 0.1ml of tuberculin (2TU, 5TU and 10TU) at either the skin of the thorax at the level of the costochrondral junction or the skin on the medial aspect of the hind limb in the thigh region and blood was collected aseptically for IFN-γ assay. Out of which the 2 dogs were found positive for TST and 3 dogs were positive for IFN-gamma assay. Moreover, X‐ray was carried out in 4 suspected dogs and only one dog had shown suspected lesion in the lung. Post-mortem was also carried out in 3 suspected tuberculosis cases brought to the Teaching Veterinary Clinical Complex (TVCC), Guwahati, Assam. An isolate of Mycobacterium was recovered from the suspected lesion test and confirmed as Mycobacterium tuberculosis by biochemical test and PCR assay. These parameters may be used for diagnosis of tuberculosis in dog.


Keywords: Dog, Mycobacterium, TST, IFN-γ assay
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How to cite this article:

Acheenta G. Barua, Koushik Kakoty, Pranjal M. Nath and Bhaben Baishya. 2019. Detection of Mycobacterium tuberculosis in Dog of Assam.Int.J.Curr.Microbiol.App.Sci. 8(5): 1283-1288. doi: https://doi.org/10.20546/ijcmas.2019.805.146