International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1283-1288

Detection of Mycobacterium tuberculosis in Dog of Assam
Acheenta G. Barua1*, Koushik Kakoty1, Pranjal M. Nath1 and Bhaben Baishya2
1Department of Veterinary Public Health,
2Teaching Veterinary Clinical Complex, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, India
*Corresponding author

A total of 52 suspected dogs with symptoms of harsh, chronic non-productive coughing, fever and anorexia were examined for tuberculosis in the Teaching Veterinary Clinical Complex, Guwahati, Assam. SIT carried out by injecting 0.1ml of tuberculin (2TU, 5TU and 10TU) at either the skin of the thorax at the level of the costochrondral junction or the skin on the medial aspect of the hind limb in the thigh region and blood was collected aseptically for IFN-γ assay. Out of which the 2 dogs were found positive for TST and 3 dogs were positive for IFN-gamma assay. Moreover, X‐ray was carried out in 4 suspected dogs and only one dog had shown suspected lesion in the lung. Post-mortem was also carried out in 3 suspected tuberculosis cases brought to the Teaching Veterinary Clinical Complex (TVCC), Guwahati, Assam. An isolate of Mycobacterium was recovered from the suspected lesion test and confirmed as Mycobacterium tuberculosis by biochemical test and PCR assay. These parameters may be used for diagnosis of tuberculosis in dog.

Keywords: Dog, Mycobacterium, TST, IFN-γ assay

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How to cite this article:

Acheenta G. Barua, Koushik Kakoty, Pranjal M. Nath and Bhaben Baishya. 2019. Detection of Mycobacterium tuberculosis in Dog of Assam.Int.J.Curr.Microbiol.App.Sci. 8(5): 1283-1288. doi:
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