Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 232-239
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.028


Comparative Evaluation of Microscopy and ELISA in Diagnosis of Cryptosporidiosis in HIV and Non-HIV Patients
A. Tejashree*, P.B. Pooja and K. Sharath Babu
Department of Microbiology, JSS Medical College and Hospital, Mysore, India
*Corresponding author
Abstract:

Cryptosporidium is one of the agents associated with diarrhoea in human immune deficiency virus patients. C. parvum is the most common parasite causing diarrhoea in immunocompromised patients. Microscopy is the diagnostic reference standard for the detection of the parasite but it is labour extensive and requires experience. A total 100 stool samples of the subjects attending ICTC and diarrhoea cases admitted in the JSS hospital from March – December 2013 were included in the study 50 subjects were HIV positive and 50 subjects were non HIV (including controls). All the samples were subjected to wet mount, Modified ZN staining and ELISA. Out of 100 samples 9(9%) were positive by ELISA, 5(5%) were positive by modified ZN only one sample was positive by all the 3 methods. Along with cryptosporidium other parasites were also detected like Isospora belli,Giradia, Cysts of Blastocystis, Eggs of Hookworm, Entamoeba histolytica. Based on our data ELISA is an useful assay for the diagnosis cryptosporidium in immunocompromised individuals.


Keywords: Cryptosporidium parvum,Immuno-compromised patients.
Download this article as Download

How to cite this article:

Tejashree, A., P.B. Pooja and Sharath Babu, K. 2017. Comparative Evaluation of Microscopy & ELISA in Diagnosis of Cryptosporidiosis in HIV and Non-HIV Patients.Int.J.Curr.Microbiol.App.Sci. 6(1): 232-239. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.028