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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1097-1103
DOI: https://doi.org/10.20546/ijcmas.2019.805.126


Immobilization of Amylase by Entrapment Method in Different Natural Matrix
Majneesh Chaudhary1, Neerja Rana1, Devina Vaidya2, Arti Ghabru1,Kavita Rana1* and Bhawna Dipta1
1Department of Basic Sciences, Microbiology Division
2Department of Food Science and Technology, Microbiology Division, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh-173230, India
*Corresponding author
Abstract:

The use of enzymes in a free form is very uneconomical because the enzymes generally cannot be recovered at the end of the reaction. These drawbacks can be overcome by immobilization of the enzyme thereby rendering it more stable and easy to recover and recycle. It is a very effective alternative for gripping the problems of instability, repetitive use and reduction in the cost of enzyme. The aim of this research was to obtain the optimum condition of the making of immobilized amylase beads using a different natural matrix. The result obtain that amylase producing isolate i.e. isolated from hot water spring and was precipitate with ammonium sulphate with specific activity of 45.29 IU/mg and 1.43 fold purification. The purified amylase was immobilized by entrapment method with 3% concentration of sodium alginate and agar with immobilization yield of 72.18% and 34.89% for isolate MW2 and 0.25% chitosan concentration gave 66.45% immobilization yield. Hence, we propose that, this method can be used to produce immobilized amylase which can be used in various areas such as diagnostics, food, medicine and cosmetics.


Keywords: Amylase, Immobilization, Purification, Entrapment, Alginate
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How to cite this article:

Majneesh Chaudhary, Neerja Rana, Devina Vaidya, Arti Ghabru, Kavita Rana and Bhawna Dipta. 2019. Immobilization of Amylase by Entrapment Method in Different Natural Matrix.Int.J.Curr.Microbiol.App.Sci. 8(5): 1097-1103. doi: https://doi.org/10.20546/ijcmas.2019.805.126