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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 993-996
DOI: https://doi.org/10.20546/ijcmas.2019.805.116


Rapid and Efficient Procedure for Genomic DNA Extraction from Trichoderma spp.
Tanvi P. Kadu*, Rajendra M. Gade, Jayant P. Rathod, Shyam Paraskar, Bhushan Malghane and Arpit Shedmake
Vasantrao Naik College of Agricultural Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Waghapur Road, Yavatmal-445001, Maharashtra, India
*Corresponding author
Abstract:

Molecular Biology experiments including gene cloning, restriction digestion, identification by 18S r-DNA requires purified and high quality genomic DNA. The major problems in DNA isolation from fungus are impurities such as polysaccharides, protein and RNAs which interfere in PCR reaction. In this study, proficient method for DNA extraction without using liquid nitrogen, CTAB or lysozyme was optimized. The method utilizes very few chemical compounds. The method involved crushing of fungal mycelia in lysis buffer containing SDS, incubation at 65ºC, extraction by chloroform, phenol and isoamyl alcohol and finally DNA precipitation by cold ethanol. The results showed DNA with high yield which can be utilized for PCR purposes.


Keywords: DNA, Trichoderma, extraction, Purity
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How to cite this article:

Tanvi P. Kadu, Rajendra M. Gade, Jayant P. Rathod, Shyam Paraskar, Bhushan Malghane and Arpit Shedmake. 2019. Rapid and Efficient Procedure for Genomic DNA Extraction from Trichoderma spp.Int.J.Curr.Microbiol.App.Sci. 8(5): 993-996. doi: https://doi.org/10.20546/ijcmas.2019.805.116