International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 993-996

Rapid and Efficient Procedure for Genomic DNA Extraction from Trichoderma spp.
Tanvi P. Kadu*, Rajendra M. Gade, Jayant P. Rathod, Shyam Paraskar, Bhushan Malghane and Arpit Shedmake
Vasantrao Naik College of Agricultural Biotechnology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Waghapur Road, Yavatmal-445001, Maharashtra, India
*Corresponding author

Molecular Biology experiments including gene cloning, restriction digestion, identification by 18S r-DNA requires purified and high quality genomic DNA. The major problems in DNA isolation from fungus are impurities such as polysaccharides, protein and RNAs which interfere in PCR reaction. In this study, proficient method for DNA extraction without using liquid nitrogen, CTAB or lysozyme was optimized. The method utilizes very few chemical compounds. The method involved crushing of fungal mycelia in lysis buffer containing SDS, incubation at 65ºC, extraction by chloroform, phenol and isoamyl alcohol and finally DNA precipitation by cold ethanol. The results showed DNA with high yield which can be utilized for PCR purposes.

Keywords: DNA, Trichoderma, extraction, Purity

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How to cite this article:

Tanvi P. Kadu, Rajendra M. Gade, Jayant P. Rathod, Shyam Paraskar, Bhushan Malghane and Arpit Shedmake. 2019. Rapid and Efficient Procedure for Genomic DNA Extraction from Trichoderma spp.Int.J.Curr.Microbiol.App.Sci. 8(5): 993-996. doi:
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