International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:2, February, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 415-424

Study of Biofilm Production and Anti-microbial susceptibility pattern of bacterial and fungal isolates from urinary catheters
Venkata Hemalatha Neeli, T. Parvathi* and Perala Balamurali Krishna
Institute: Department of Microbiology, Andhra Medical College, Visakhapatnam, India
*Corresponding author

A biofilm is an accumulation of microorganisms embedded in an exo-polysaccharide matrix of microbial and host origin called Polysaccharide Intercellular Adhesin (PIA). They pose a great threat to indwelling devices causing slow, persistent infections; thereby interfering with antibiotic therapy. This study was carried out to isolate and identify the bacteria and fungi from the tips of urinary catheters, to determine their anti-microbial susceptibility patterns and to detect their ability to produce biofilm by three different methods. 132 urinary catheter tips were collected from patients admitted into various wards and the organisms isolated were subjected to three different tests to detect their ability to produce biofilms, i.e., a) Tube Method, b) Congo Red Agar method and c) Tissue Culture Plate method. 49 samples showed culture positivity; out of which, 18 produced biofilms. TCP method detected 42% biofilm producers whereas Tube method and Congo red agar method detected 32% and 26% respectively. Antimicrobial susceptibility testing was done and increased resistance was shown by biofilm producers. The information on the capacity of a clinical isolate to produce biofilm would help a clinician to evaluate the measure of its virulence and devise an appropriate treatment plan for the patient

Keywords: Biofilm,Urinary catheter tips,Tube Method, Congo Red Agar method,Tissue Culture Plate method

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How to cite this article:

Venkata Hemalatha Neeli, T. Parvathi and Perala Balamurali Krishna. 2016. Study of Biofilm Production and Anti-microbial susceptibility pattern of bacterial and fungal isolates from urinary catheters.Int.J.Curr.Microbiol.App.Sci. 5(2): 415-424. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.