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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 42-50
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.006


Plasmid Mediated Quinolone Resistance Determinants among Nosocomial Clinical Pseudomonas aeruginosa Isolates

Mohammed A. Saleh1* and Mohammed M. Balboula2

1Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University,New Damietta, Egypt
2Department of General Surgery, Faculty of Medicine, Al-Azhar University,New Damietta, Egypt
*Corresponding author
Abstract:

Quinolones are among the most commonly utilized antimicrobials for treatment of infections caused by P. aeruginosa. Plasmid mediated quinolone resistance (PMQR) is an expanding clinical concern worldwide with fluctuating prevalence rates. Spread of PMQR determinants along with other resistance mechanisms to P. aeruginosa clinical isolates would constrain the adequacy of quinolones in treatment of Pseudomonas infections. This study was designed to investigate the incidence of PMQR determinants among nosocomial P. aeruginosa isolates. A total of 110 clinical P. aeruginosa were collected over a period from January 2012 to October 2015frominpatients in Al-Azhar University Hospital, New Damietta, Egypt. Testing of isolates for resistance to four quinolones was performed using Kirby-Bauer disc diffusion method. Multiplex PCR was carried out for screening of the eight PMQR genes(qnrgenes (A,B,C,D,S), aac(6´)-Ib-cr, qepA and oqxAB).Out of the 110 P. aeruginosa isolates tested, 47(42.7%)isolates were sensitive to the 4 tested quinolones while 63(57.3%) were resistant to one or more of them. Testing of isolates for PMQR genes showed that 5(4.5%) harbored qnr genes, 2(1.8%) were qnr B positive and 3(2.7%) were qnr S positive. Isolates were negative for the other tested PMQR genes. Generally, there is no statistically significant association between quinolone resistance and the presence of PMQR genes. This study highlighted diverse PMQR genes and their prevalence among P. aeruginosa isolates. The complex epidemiology of PMQR strains needs to be further studied in order to design measures to control their spread.


Keywords: Plasmid mediated quinolone Resistance, Pseudomonas aerogonisa.
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How to cite this article:

Mohammed A. Saleh and Mohammed M. Balboula. 2017. Plasmid Mediated Quinolone Resistance Determinants among Nosocomial Clinical Pseudomonas aeruginosa Isolates.Int.J.Curr.Microbiol.App.Sci. 6(1): 42-50. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.006