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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 389-396
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.044


Immobilization of Bacilli in Various Matrices to Enhance the Utilization of Keratinase and its Comparison
Ch.M.Kumari Chitturi* and V.V.Lakshmi
Department of Applied Microbiology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati-517502, India
*Corresponding author
Abstract:

Bioconversion of insoluble Keratin-rich waste in to soluble value added product is an alternative way for recycling recalcitrant waste from poultry and leather industries. Microbial keratinases are more prominent which target the hydrolysis of keratin. Immobilization of the cell/ enzyme is an approach known to increase the application potential of biocatalysts for recycling of the cells and increase the efficiency of enzyme utilization. In present investigation effect of various matrices to immobilize Bacillus cells for the production of Keratinase was studied. Matrices like sodium alginate, polyacrylamide, agar-agar and gelatin were used. Keratinase activity of immobilized alginate beads was significantly higher (424 - 385 KU/ml) when compared to polyacrylamide (237-208KU/ml), gelatin (128-78KU/ml), agar-agar blocks (237-103KU/ml), both in terms of activity and stability of beads. Sodium alginate immobilized Bacilli were efficient in the production of Keratinase bringing 100% degradation of feather in 4-5 days similar to free cells. Further cells could be recycled up to 3 batches over 21 days, especially sodium alginate beads are repeatedly used which can withstand disintegration of bead stability making the process economical and viable.


Keywords: Immobilization, Bacilli, Matrices, Keratinase activity, Feather waste, Sodium alginate, Gelatin.
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How to cite this article:

Kumari Chitturi, Ch.M., and Lakshmi, V.V. 2016. Immobilization of Bacilli in Various Matrices to Enhance the Utilization of Keratinase and its Comparison.Int.J.Curr.Microbiol.App.Sci. 5(2): 389-396. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.044