International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:2, February, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 380-388

Antimicrobial Assay and Minimum Inhibitory Concentration Values of Cistanche tubulosa
Najeeb Ullah1, Abdur Rehman2, Sohail Ahmad4*, Noreen Samad1, Hina Andaleeb1, Waqas Ahmad2, Khadim Ahmad2, Muhammad Haroon2, Shahid Maqbool1, Adeela Altaf3 and Naima Raza3
1Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Punjab, Pakistan

2Department of Chemistry, Kohat University of Science and Technology, Kohat, 26000, KPK, Pakistan
3Department of Environmental Sciences, Bahauddin Zakariya University,
Multan 60800, Punjab,   Pakistan
4Department of Chemistry, Qurtuba University of Science and Information Technology Peshawar, Peshawar 25120, Pakistan
*Corresponding author

The study describes the extraction, fractionation and antimicrobial activity of Cistanche tubulosa. The plant crude methanolic extract and all sub fractions (n-hexane, chloroform, ethyl acetate, n-butanol and aqueous) were subjected to antibacterial and antifungal activity. Agar well diffusion method was used for antimicrobial activities. Methanolic extracts of C. tubulosa show widest zone of inhibition (8mm) and ethyl acetate (7mm) against P. mirabilis using agar well diffusion. Hexane fraction show interesting results of abrupt change in inhibition showing 7mm at 4mg/mL and 1.5mm at 2mg/mL concentration. We got some interesting results from chloroform fraction against K. pneumoniae showing 2mm inhibition at 2mg/mL and 4mm at 1mg/mL concentration. Same against P. mirabilis by hexane fraction showing 3.1mm at 2mg/mL and 4mm at 1mg/ml concentration. The aqueous fraction show inhibition of 7.1mm against E.coli. In antifungal activity methenaol, aqueous and hexane fractions show no inhibition at 1mg/mL concentrataion against A.fumigatus. So the MIC values of these three fractions is 2mg/mL against A.fumigatus. Highest activity fraction was butanol which gives wider zone of 7mm against A. fumigantus. Whereas the same MIC value of methanol extract is 2mg/ml against A.niger. The results established a good support for the use of C. tubulosa in traditional medicine.

Keywords: MIC, Phytochemicals, Antimicrobial agents, Cistanche tubulosa Inhibition

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Najeeb Ullah, Abdur Rehman, Sohail Ahmad, Noreen Samad, Hina Andaleeb, Waqas Ahmad, Khadim Ahmad, Muhammad Haroon, Shahid Maqbool, Adeela Altaf and Naima Raza. 2016. Antimicrobial Assay and Minimum Inhibitory Concentration Values of Cistanche tubulosa.Int.J.Curr.Microbiol.App.Sci. 5(2): 380-388. doi:
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