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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1215-1229
DOI: https://doi.org/10.20546/ijcmas.2019.804.140


Trichoderma asperellum Mediated Synthesis of Silver Nanoparticles: Characterization and its Physiological Effects on Tea [Camellia sinensis (L.) Kuntze var. assamica (J. Masters) Kitam.]
Afrina Ara Ahmed* and Pranab Dutta
Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam, India
*Corresponding author
Abstract:

Silver nanoparticles (Ag NPs) were synthesized using culture filtrate of fungal antagonist Trichoderma asperellum for which silver nitrate was used as the precursor and were characterized by using UV-Vis spectroscopy, DLS, Zeta sizer, TEM and EDX. The physiological effects of Ag NPs on the host crop i.e. tea (clone-TV21) was evaluated at 100% concentration by introducing into the host with five different treatment methods viz. cutting treatment (one leaf bud cutting), injection method, foliar spray, soil application and seedling root dip treatment with 10 replicates for each. Observation on changes in chlorophyll content, moisture content, relative water content, total soluble sugar, total protein, lipid peroxidation (MDA content), secondary metabolites viz. phenol, alkaloid, and flavonoid were analyzed after 45 days of the treatment. Results showed that silver nanoparticles can induce the plants in increasing all the studied physiological parameters. Out of all the five treatments, foliar spray followed by seedling root dip treatment was found to be the best treatment for establishing silver nanoparticles in the plant system with maximum positive effects on all the parameters compared to other treatments and as control.


Keywords: Silver nanoparticles, Trichoderma asperellum, Physiological effects, Tea
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How to cite this article:

Afrina Ara Ahmed and Pranab Dutta. 2019. Trichoderma asperellum Mediated Synthesis of Silver Nanoparticles: Characterization and its Physiological Effects on Tea [Camellia sinensis (L.) Kuntze var. assamica (J. Masters) Kitam.].Int.J.Curr.Microbiol.App.Sci. 8(4): 1215-1229. doi: https://doi.org/10.20546/ijcmas.2019.804.140