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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 660-671
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.074


Characterization of Carbapenem Resistant Acinetobacter baumannii causing Ventilator associated Pneumonia in ICUs of Zagazig University Hospitals, Egypt
Ghada E. Amr1* and Ghada M. Abdel Razek2
1Departments of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt
2Anaesthesia and Intensive Care, Faculty of Medicine, Zagazig University, Egypt
*Corresponding author
Abstract:

Ventilator associated pneumonia (VAP) affects approximately 30% of intubated mechanically ventilated patients in intensive care units (ICUs) worldwide. Acinetobacter baumannii causing VAP is often resistant to a wide variety of antibiotics including carbapenems that have been considered the drug of choice for the treatment for this infection. To characterize A. baumannii as a cause of VAP in our ICUs and to study its incidence, antimicrobial resistant profile and to investigate the presence of carbapenem hydrolyzing class D b-lactamase genes as a cause of carbapenem resistance among the studied isolates. A total of 44 A. baumannii isolates were recovered from 135 endotracheal aspirate samples of VAP patients. A. baumannii were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry and its antimicrobial  susceptibility was investigated by VITEK 2 instrument and E test, class D b- lactamase genes were investigated by multiplex PCR technique. All A. baumannii isolates were multidrug resistant;75% were resistant to carbapenems while colistin remains the most active compound among the studied isolates with sensitivity rate of 93.2%. Multiplex PCR results showed that allA. Baumannii isolates were positive for blaOXA-51- like gene while 69.7% of carbapenems resistant isolates were positive for blaOXA-23 like gene,blaOXA-58 and blaOXA-24 like genes were not detected in any of the studied isolates. This study highlights the emergence of carbapenem resistant A. baumannii as a cause of VAP in our ICUs that was mostly due the presence of blaOXA-23 like gene.


Keywords: Acinetobacter baumannii,blaOXA-23,VAP.
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How to cite this article:

Ghada E. Amr and Ghada M. Abdel Razek. 2016. Characterization of Carbapenem Resistant Acinetobacter baumannii causing Ventilator associated Pneumonia in ICUs of Zagazig University Hospitals, EgyptInt.J.Curr.Microbiol.App.Sci. 5(12): 660-671. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.074