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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1378-1392
DOI: https://doi.org/10.20546/ijcmas.2019.803.162


Faster Chemical Methods to Determine Genetic Purity in Pigeon Pea (Cajanus cajan (L.) Mill sp.)
Rakesh C. Mathad1*, A.S. Channaveeraswamy2, D. Vinod Kumar3,V. Rudra Naik4 and S.A. Ashtaputre5
1Department of Seed Science and Technology, College of Agriculture, UAS, Dharwad, India
2Head and Farm Superintendent, ARS, Annigeri, India
 3Principal Scientist, IGFRI, Regional Station, Dharwad, India
4Genetics and Plant Breeding and Chief Scientific Officer, UAS, Dharwad, India
5Plant Pathology and Assistant Director of Research, UAS, Dharwad, India
*Corresponding author
Abstract:

The present investigation was carried out to find faster chemical methods to determine genetic purity in ten pigeon pea varieties. The faster methods are Ferrous Sulphate test, Sodium hydroxide test, peroxidase test, seedling growth response to GA3, kinetin and 2-4D. Histogram using pixel luminance in ferrous sulphate test based on the grey scale produces values ranging from 17377 to 90616 which can be used to distinguish pigeon pea varieties. Similarly, sodium hydroxide test produced six coloured palette based on RGB values can distinguish varieties in pigeon pea. Also in peroxidase test, values for all ten pigeon pea varieties based on absorbance (0.252-2.372), peroxidase enzyme activity (0.791-7.442 IU/mg) and peroxidase enzyme volume (1.581-14.883 U/g) can be used for distinguishing varieties. Among the seedling growth response tests, in GA3 test two varieties Asha (-2) and GC-11-39 (-6) were not responded and were recorded lowest values than the control. But remaining varieties can be distinguished as medium to high response types. Also in kinetin test two varieties not responded (GRG-811 and Asha) and all the other varieties can be distinguished based on this test. In case of 2-4D test all the varieties were recorded as susceptible. The co-efficient of variation for all the three tests based on the increase in coleoptile length over the control was ranged from 0.20 (Kinetin test) to 0.37 (GA3 test).


Keywords: Ferrous sulphate test, Sodium Hydroxide test, Peroxidase test, Genetic purity test, Seedling growth response, Descriptors
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How to cite this article:

Rakesh C. Mathad, A.S. Channaveeraswamy, D. Vinod Kumar, V. Rudra Naik and Ashtaputre, S.A. 2019. Faster Chemical Methods to Determine Genetic Purity in Pigeon Pea (Cajanus cajan (L.) Millsp.).Int.J.Curr.Microbiol.App.Sci. 8(3): 1378-1392. doi: https://doi.org/10.20546/ijcmas.2019.803.162