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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1228-1236
DOI: https://doi.org/10.20546/ijcmas.2019.803.145


Effect of 2, 4 D and EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032
Praveen Kona1*, M. Hemanth Kumar2, K.H.P. Reddy3, D.M. Reddy3, N.P. Eswar Reddy4, P. Latha4 and M.S. Balaji2
1Crop Improvement Unit, Directorate of Groundnut Research, Junagadh- 362001, India
2 Department of Genetics and Plant Breeding, Agricultural Research Station, Perumallapalle, India
3Department of Genetics and Plant Breeding, S. V. Agricultural College, Tirupati, 517 502, India
4Institute of Frontier Technologies, RARS, Tirupati, 517 502, India
*Corresponding author
Abstract:

The present studies were carried out at Agricultural Research station, Perumallapalle to establish an efficient system for in vitro regeneration of sugarcane cultivar, Co86032 using chemical mutagens; Ethyl methane sulphonate (EMS) and 2, 4-dichlorophenoxy acetic acid (2, 4 D). Young leaf rolls were used as explants for callus induction on MS medium containing different concentrations of EMS (0.6 µM l-1, 0.8 µM l-1 and 1.0 µM l-1) and 2, 4-D (4mg l-1, 5mg l-1 and 6 mg l-1). Among the different concentrations, Co86032 had taken minimum number of days (11.89) for callus induction with 2, 4 D @ 4mg l-1, recording maximum mean callus size of 2.19 cm with high callus induction frequency (96.30%) and maximum mean number of explants inducing callus (2.78) in addition with superior response in shooting, rooting and hardening characters than EMS.EMS and 2, 4 Dat higher concentration (1.0 µM l-1 and 6mg l-1, respectively) had inhibitory effect on callusing, shooting and rooting and produced morphological traits variation which can be exploited in breeding programs.


Keywords: Sugarcane, in vitro, EMS, 2, 4 D, Callus
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How to cite this article:

Praveen Kona, M. Hemanth Kumar, K.H.P. Reddy, D.M. Reddy, N.P. Eswar Reddy, P. Latha and Balaji, M.S. 2019. Effect of 2, 4 D & EMS On in vitro Regeneration in Sugarcane Cultivar, Co86032.Int.J.Curr.Microbiol.App.Sci. 8(3): 1228-1236. doi: https://doi.org/10.20546/ijcmas.2019.803.145