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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 603-608
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.065


A Study on the Rate of vacA Genotypes of Helicobacter pylori in Gastric Biopsies obtained from Patients with Symptomatic Upper GIT Disorders
Balamurugan Rangasamy1*, Saleem Mohammed Ali2, Kaviraj Mahadevan1,  and R. Marudhavanan3
1Central Research Laboratory, Sri Manakula Vinayagar Medical College and Hospital, Puducherry, India
 2Department of Microbiology, Sri Manakula Vinayagar Medical College and Hospital, Puducherry, India
3Department of Surgery, Sri Manakula Vinayagar Medical College and
Hospital, Puducherry, India
*Corresponding author
Abstract:

Vacoulating cytotoxin (vacA) gene is one of the most studied virulent genes of Helicobacter pylori which plays a major role in causing inflammation in the human gastric epithelium. We have studied the rate of vacA genotypes of H.pylori in gastric biopsy samples from patients with upper GIT disorders. Antral biopsies (60 nos.) were collected from patients undergoing upper GIT endoscopy at our hospital. Rapid urease Test and Dilute Carbol Fuchsin staining were used to detect H.pylori from the biopsy tissue. The rate of vacA genotypes were studied by PCR, targeting the intermediate region (vacAi) of the vacA gene. 31 of the 60 biopsies were positive for H.pylori by RUT and DCF. vacAi region of the vacA gene was amplified from 9 (29%) of the H.pylori positive cases. The rate of vacA genotypes determined in symptomatic upper GI disorders is very low. This study reveals that vacA is not the one and only factor for H.pylori induced inflammation and further consequences. Gene diversity and combination of virulent genes and their association with other risk factors should be studied to divulge the role virulent genes in H.pylori.


Keywords: Helicobacter pylori,VacA, GIT disorders,Rapid urease Test (RUT).
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How to cite this article:

Balamurugan Rangasamy, Saleem Mohammed Ali, Kaviraj Mahadevan and R. Marudhavanan. 2016. A Study on the Rate of vacA Genotypes of Helicobacter pylori in Gastric Biopsies obtained from Patients with Symptomatic Upper GIT Disorders.Int.J.Curr.Microbiol.App.Sci. 5(12): 603-608. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.065