Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1-6
DOI: https://doi.org/10.20546/ijcmas.2019.803.001


OprD Protein Profile of Pseudomonas aeruginosa Isolates Resistant to Imipenem from Patients in Khartoum State – Sudan
Somaia Alsir1* and Omeima Salih2
1School of Pharmacy - Ahfad University for Women, Omdurman, Sudan
2School of Health Sciences – Ahfad University for Women, Omdurman, Sudan
*Corresponding author
Abstract:

In Sudan, Pseudomonas aeruginosa is the most antibiotics resistant bacteria isolated among other bacterial strains of clinical impact. Carbapenems, such as imipenem are often used as last resort antibiotics for the treatment of multidrug-resistant Pseudomonas aeruginosa infection. The study was performed to evaluate the OprD porin protein profile among clinical isolates of Pseudomonas aeruginosa from urine samples. Fifty six clinical isolates of Pseudomonas aeruginosa were collected from different hospitals in Khartoum State. Imipenem susceptibility test was determined by the disk diffusion method. Carbapenems production was confirmed by Disk Enhancement Test (DET) and Combined disk test (CDT) Imipenem-Cloxacillin. The protein profile of the isolates was determined by SDS-poly acrylamide gel electrophoresis. Seventy two percent (72%) of the isolates were resistant to imepenim and about forty percent (39.9%) of the imepenium resistant isolates were metalllobeta lacatamases producers. However, all resistant isolates showed OprD prion protein deficiency. This concludes the importance of OprD protein expression in increasing the sensitivity of Pseudomonas aeruginosa to antibiotics.


Keywords: Pseudomonas aeruginosa, Clinical isolates, OprD, Sudan
Download this article as Download

How to cite this article:

Somaia Alsir and Omeima Salih. 2019. OprD Protein Profile of Pseudomonas aeruginosa Isolates Resistant to Imipenem from Patients in Khartoum State – Sudan.Int.J.Curr.Microbiol.App.Sci. 8(3): 1-6. doi: https://doi.org/10.20546/ijcmas.2019.803.001