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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3291-3298
DOI: https://doi.org/10.20546/ijcmas.2019.802.384


Isolation, Characterization and Molecular Identification of Culturable Gut Bacteria in Diamondback Moth, Plutella xylostella (Linnaeus)
W. Vijaykumar1, R. Muthuraju1, B. Shivanna2, K. Archana1 and B.S. Nalini1
1Department of Agricultural Microbiology
2Department of Agricultural Entomology, University of Agricultural Sciences, Bengaluru-560065, India
*Corresponding author
Abstract:

Diamondback moth, Plutella xylostella is a major pest of cruciferous crops worldwide and it has developed resistance to almost all synthetic insecticides. It was known to harbour microorganisms which play important role in growth and development of the host. In the present study bacterial strains were isolated from third instar larvae of P. xylostella collected from Sugatur, Kolar District of the state Karnataka. Morphological and Biochemical characterization were done, among them most of the bacterial isolates were gram negative and negative for some biochemical tests. Further, total bacterial genomic DNA was extracted from the bacterial isolates and amplified using PCR with 16S rRNA primers (expected size 1000 bp). Ten different bacterial isolates were isolated and five were identified at genus level such as Serratia marcescens (DBM1 and DBM9), Serratia nematodiphila (DBM2), Serratiasp. (DBM3) and Myroidesodoratus (DBM4). The Serratia spp. is the most predominant bacterial isolate in this region. These studies suggested that a combination of molecular and traditional culturing methods can be effectively used to analyze and determine the diversity of gut microflora. These bacterial strains may play important roles in growth and development of P. xylostella.


Keywords: Diamondback moth (DBM), Endosymbionts, Catalase and IMVIC tests, 16S rRNA, Serratia spp
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How to cite this article:

Vijaykumar, W., R. Muthuraju, B. Shivanna, K. Archana and Nalini, B.S. 2019. Isolation, Characterization and Molecular Identification of Culturable Gut Bacteria in Diamondback Moth, Plutella xylostella (Linnaeus).Int.J.Curr.Microbiol.App.Sci. 8(2): 3291-3298. doi: https://doi.org/10.20546/ijcmas.2019.802.384