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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3049-3058
DOI: https://doi.org/10.20546/ijcmas.2019.802.357


Analysis of Quinolones Residues in Milk using High Performance Liquid Chromatography
Priyanka, Vijay J. Jadhav*, Sneh Lata Chauhan and S.R. Garg
Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana- 125004, India
*Corresponding author
Abstract:

In the present study, High Performance Liquid Chromatography with Ultra-Voilet detector (HPLC-UV) technique was standardized and validated for the detection and quantitation of quinolones antimicrobial residues viz. enrofloxacin, norfloxacin and ciprofloxacin from milk. The standardization procedure showed that the values for the system precision (% RSD) for both the analytes was 11% for area and <0.9% for retention time), linearity (r2>0.98), specificity and accuracy (70-110%) and precision (<10%) were within accepted range and demonstrated system suitability for analysis of milk samples. The standardized and validated method was applied for the detection of quinolones residues from 100 randomly milk samples collected from local market of Hisar (Haryana). Mean concentrations of norfloxacin and enrofloxacin antimicrobial residues in market milk samples were 3.54 and 2.02 µg/kg, respectively. A total of 8 samples were found to be containing quinolone antimicrobial residues. Comparison of antimicrobial concentration in each positive sample of milk with international MRLs showed that, none of the three antimicrobial was responsible for violations of set residue limits. It was concluded that milk is significant source of antimicrobial residues.


Keywords: HPLC, Quinolones, Milk, Antimicrobial residues, MRL
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How to cite this article:

Priyanka, Vijay J. Jadhav, Sneh Lata Chauhan and Garg, S.R. 2019. Analysis of Quinolones Residues in Milk using High Performance Liquid Chromatography.Int.J.Curr.Microbiol.App.Sci. 8(2): 3049-3058. doi: https://doi.org/10.20546/ijcmas.2019.802.357