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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 437-451
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.048


Molecular Changes and Gene Expression of Glutathione Peroxidase in Adrenal Gland during Experimental Fluorosis 
Shashi* and Neha Sharma
Department of Zoology and Environmental Sciences, Punjabi University, Patiala-147002, Punjab, India
*Corresponding author
Abstract:

Fluorosis is known to cause several metabolic and degenerative disorders and has alarming upsurge in the recent years which is a matter of deep concern and need intense research. The gene expression of the anti-oxidant enzyme glutathione peroxidase was studied in adrenal gland of Wistar albino rats treated with sodium fluoride at the dose of 100, 200, 300 ppm/kg b.w. orally for 40 days. The levels of DNA, RNA and proteins in adrenal gland were lower and showed negative co-relation with fluoride. RT-PCR analysis showed a decrease in glutathione peroxidase gene expression in response to fluoride intoxication, suggesting a role for post translational modification in altering the activities of this enzyme. Western-blot analysis showed that due to oxidative stress protein was expressed at 24-kDa in all fluoride treated groups. Random Amplification of Polymorphic DNA marker were generated via the polymerase chain reaction using shorter primers of an arbitrary sequence. With high concentration of fluoride rapid gel images showed greater variation then at low dose which was due to certain mutation which change the primer binding site.


Keywords: Adrenal gland,Gene expression, Glutathione peroxidase,RT-PCR,Sodium fluoride
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How to cite this article:

Shashi, A., and Neha Sharma. 2016. Molecular Changes and Gene Expression of Glutathione Peroxidase in Adrenal Gland during Experimental Fluorosis.Int.J.Curr.Microbiol.App.Sci. 5(12): 437-451. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.048