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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2696-2701
DOI: https://doi.org/10.20546/ijcmas.2019.802.315


PCR Detection of blaIMP Gene in Metallo-β-Lactamase Resistant E. coli Isolated from Clinical Samples
Rahul Narasanna, Siddarth Surwonse, Manjunath Chavadi and Chandrakanth Kelmani*
Department of Biotechnology Gulbarga University Kalaburagi, India
*Corresponding author
Abstract:

PCR detection of blaIMP gene in Imipenem resistant E. coli is the aim ot this study. Total of n=66 of n=23 Imipenem resistant E. coli were selected for the present study based on the drug resistance pattern and their MIC to Imipenem. Phenotypic detection of MBL production was carried out and subsequently, PCR amplification of blaIMP was carried out. Among selected (n=23) Imipenem resistant E. coli (IREC), 100% (n=23) strains were positive for Imipenem mediated MBL production. Twenty-three MBL positive IREC isolates were shown presence of Plasmid DNA, with size measured about approx. 6kb. PCR amplification of blaIMP gene codes for Imipenem mediated MBL production yielded amplified blaIMP gene product with amplicon size of 189 bp. The presence blaIMP gene also contributes for MBL production which makes organisms to show reduced susceptibility against Imipenem antibiotic


Keywords: Imipenem resistant E. coli, MBL, blaIMP
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How to cite this article:

Rahul Narasanna, Siddarth Surwonse, Manjunath Chavadi and Chandrakanth Kelmani. 2019. PCR Detection of blaIMP Gene in Metallo-β-Lactamase Resistant E. coli Isolated from Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 8(2): 2696-2701. doi: https://doi.org/10.20546/ijcmas.2019.802.315