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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 376-382
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.041


In-vitro Study of Somaclonal Variation in Chlorophytum borivilianum
Vinai Kumar1* and Chander Prabha2
1Tissue Culture Lab., Department of Botany, Patna University, Patna Bihar, India
2Department of Botany, Patna University, Patna Bihar, India
*Corresponding author
Abstract:

Variations are genetically induced either in vivo or in vitro growth.  The objective of the present investigation was to evaluate the effect of variations under in vitro growth in the Chlorophytum borivilianum an endangered medicinal herb. The chemically defined nutrient medium was act as stimulants to the explant and the growth response of regenerates were assessed. The MS media in combination with different growth regulators & pH (chemical factors) and some of the physical factors (external microenvironments i.e. variations in relative humidity, temp. and light/dark periods) was responsible for variations. The in vitro treatment, including different concentrations of the growth regulators were inducing agent. The long time in culture condition produced the greatest probability to initiate variations in clone lines under controlled environment. Three types of regenerates were recorded i.e. normal, vigorous, and dwarf. These variates were marked on the basis of visual score at the time of rooting. Medium compositions were act as elicitor and responsible for variations during the in-vitro multiplications. Due to impact of medium signal changes were recorded in the clones of the same propagules. The obtained mutant variety through in vitro selection was considered as somaclonals. These variations were recorded by visual scoring in their morphology. The variant shows morphological variations from their parental plant.


Keywords: Chlorophytum borivilianum, medium signals, in vitro,somaclonalvariates,regenerates.
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How to cite this article:

Vinai Kumar and Chander Prabha. 2016. In-vitro Study of Somaclonal Variation in Chlorophytum borivilianum.Int.J.Curr.Microbiol.App.Sci. 5(12): 376-382. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.041