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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2108-2117
DOI: https://doi.org/10.20546/ijcmas.2019.802.244


Standardization of in vitro Hardening Strategies for Tissue Cultured Wine Grape (Vitis vinifera L) Genotypes
Rahul Dev1*, S.K. Singh2, Vishambhar Dayal2, Kamlesh Kumar3 and Traloki Singh4
1CAZRI, RRS, Kukma-Bhuj, Gujarat 370 105
 2ICAR Research Complex for NEH Region, Mizoram Centre
 3CIAH Bikaner
4SMSCAZRI, KVK, Kukma-Bhuj, Gujarat 370 105
*Corresponding author
Abstract:

A study was carried out to establish efficient hardening strategy for in vitro raised plantlets of four grape genotypes. In vitro grown plantlets of Pusa Navrang, Hybrid 76-1 (Hur x Cardinal), Pearl of Csaba and Julesky Muscat were hardened in vitro using hardening medium consisting coco peat + vermiculite + perlite (2:1:1) ratio and two different types of hardening pots viz. glass jars with polypropylene cap (GJPP) and plastic pots with polyethylene cover (PPPC). The glass jar containing coco peat + vermiculite + perlite (2:1:1) was found the most effective strategy for hardening in vitro raised plantlets which gave the highest survival (85.97%) and earliest duration for acclimatization of plantlets (23.56 days). Plastic pot with the coco peat + vermiculite + perlite (2:1:1) (T2) was found non-significant since it required a longer duration for acclimatization (27.93) and also giving lower survival rate (63.46%).


Keywords: Vitis vinifera, In vitro hardening, Acclimatization, Polyethylene
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How to cite this article:

Rahul Dev, S.K. Singh, Vishambhar Dayal, Kamlesh Kumar and Traloki Singh. 2019. Standardization of in vitro Hardening Strategies for Tissue Cultured Wine Grape (Vitis vinifera L) Genotypes.Int.J.Curr.Microbiol.App.Sci. 8(2): 2108-2117. doi: https://doi.org/10.20546/ijcmas.2019.802.244