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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 961-965
DOI: https://doi.org/10.20546/ijcmas.2019.802.111


Comparison between Conventional and in-situ Chromosome Doubling Method in Triticum Durum x Aegilops tauschii Crosses
S.R. Cambay1*, P. Srivastava2, S.K. Sandhu2, N.S. Bains2 and Maneet Rana1
1Division of Genetics, IARI, New Delhi, 110012, India
2Department of Plant Breeding & Genetics, PAU, Ludhiana, 141012, India
*Corresponding author
Abstract:

In this investigation two different methods using aqueous solutions of colchicine were used to treat F1 tillers from various Triticum durum x Aegilops tauschii crosses in order to artificially induce chromosome doubling. Treatment of crown root region (uproot) was found effective compared to in-situ (tip) method using treatment of apical meristems of F1 tillers at 2-3 tiller stage. The aqueous solution of colchicine was administered at two concentrations viz., 0.05 and 0.075% in both the methods. The 0.05% colchicine solution was found more effective as more doubled seed was obtained.


Keywords: in-situ Chromosome, Triticum Durum Aegilops tauschii
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How to cite this article:

Cambay, S.R., P. Srivastava, S.K. Sandhu, N.S. Bains and Maneet Rana, 2019. Comparison between Conventional and in-situ Chromosome Doubling Method in Triticum Durum x Aegilops tauschii Crosses.Int.J.Curr.Microbiol.App.Sci. 8(2): 961-965. doi: https://doi.org/10.20546/ijcmas.2019.802.111