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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 941-947
DOI: https://doi.org/10.20546/ijcmas.2019.802.108


Diagnosis of Bovine Sarcocystosis by Conventional Methods
Rasmita Panda*, Manchukonda Udaya Kumar, G.S. Sreenivasa Murthy and Y.N. Reddy
Department of Veterinary Parasitology, College of Veterinary Science, PVNR TVU, Rajendranagar, Hyderabad-500 030, Telangana, India
*Corresponding author
Abstract:

Sarcocystis species are considered as one of the most prevalent parasites of bovines. Sarcocysts occur either macroscopically or microscopically in the tissues of bovine intermediate hosts. Sarcocystosis being an occult infection, most of the time diagnosis in intermediate host by clinical symptoms is unreliable. In this study, the macrosarcocysts were observed in the infected muscle sample by naked eye examination. Suspected muscle samples were examined by muscle squash technique which showed the presence of microsarcocysts in the muscle tissue and bradyzoites were recovered through muscle digestion technique. The detailed morphology of the recovered bradyzoites was studied by Giemsa, Leishman’s and Diff Quick staining which revealed banana shaped bradyzoites with a clear nucleus towards the posterior end. The trypan blue staining indicated live (colourless) and dead (blue) bradyzoites and the gliding movement of the bradyzoites was recorded.


Keywords: Bovine, Sarcocystis, Bradyzoites, Staining
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How to cite this article:

Rasmita Panda, Manchukonda Udaya Kumar, G.S. Sreenivasa Murthy and Reddy, Y.N. 2019. Diagnosis of Bovine Sarcocystosis by Conventional Methods.Int.J.Curr.Microbiol.App.Sci. 8(2): 941-947. doi: https://doi.org/10.20546/ijcmas.2019.802.108